Prostate Specific Antigen antibody (AA 25-261)

Catalog No. ABIN3043283

The Rabbit Polyclonal anti-Prostate Specific Antigen antibody has been validated for IHC and WB. It is suitable to detect Prostate Specific Antigen in samples from Human.

Quick Overview for Prostate Specific Antigen antibody (AA 25-261) (ABIN3043283)

Target: Prostate Specific Antigen (PSA)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prostate Specific Antigen antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-Prostate Specific Antigen Antibody

Binding Specificity: AA 25-261

Purpose: Anti-Prostate Specific Antigen/KLK3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Prostate Specific Antigen/KLK3 Antibody Picoband® (ABIN3043283). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PSA recombinant protein (Position: I25-P261). Human PSA shares 54% amino acid (aa) sequence identity with rat PSA.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/mL, Human
1. Balk SP, Ko YJ, Bubley GJ (January 2003). "Biology of prostate-specific antigen". J. Clin. Oncol. 21 (2): 383-91. 2. Catalona, W. J., Richie, J. P., Ahmann, F. R., Hudson, M. A., Scardino, P. T., Flanigan, R. C., Dekernion, J. B., Ratliff, T. L., Kavoussi, L. R., Dalkin, B. L. (1994). "Comparison of digital rectal examination and serum prostate specific antigen in the early detection of prostate cancer: Results of a multicenter clinical trial of 6,630 men". The Journal of urology 151 (5): 1283-1290. 3. Lilja H (November 2003). "Biology of prostate-specific antigen". Urology 62 (5 Suppl 1): 27-33. doi:10.1016/S0090-4295(03)00775-1.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Alternative Name: KLK3

Background:

Synonyms: Prostate-specific antigen,PSA,3.4.21.77,Gamma-seminoprotein,Seminin,Kallikrein-3,P-30 antigen,Semenogelase,KLK3,APS,

Tissue Specificity: Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells. .

Background: Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a glycoprotein enzyme encoded in humans by the KLK3 gene. KLK3 is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. This gene is mapped to 19q13.33. KLK3 is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. KLK3 is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely. It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm. It is not a unique indicator of prostate cancer, but may also detect prostatitis or benign prostatic hyperplasia.

Sequence Similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.

Molecular Weight: 29 kDa

Gene ID: 354

UniProt: P07288

Pathways: Complement System