SCARB1 antibody (C-Term)

Catalog No. ABIN3043322

The Rabbit Polyclonal anti-SCARB1 antibody is suitable to detect SCARB1 in samples from Rat and Mouse. It has been validated for WB and IHC.

Quick Overview for SCARB1 antibody (C-Term) (ABIN3043322)

Target: SCARB1 (Scavenger Receptor Class B, Member 1 (SCARB1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCARB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SCARB1 Antibody

Binding Specificity: C-Term

Purpose: Anti-Scavenging Receptor SR-BI/SCARB1 Antibody

Sequence: KKGSQDKEAI QAYSESLMSP AAKGTVLQEA KL

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat sequence by one amino acid.

Characteristics: Anti-Scavenging Receptor SR-BI/SCARB1 Antibody. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse SCARB1, different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SCARB1

Target: SCARB1 (Scavenger Receptor Class B, Member 1 (SCARB1))

Alternative Name: Scarb1

Background:

Background: Scavenger receptor class B member 1 (SRB1), also known as SR-BI, is a protein that in humans is encoded by the SCARB1 gene. SR-BI functions as a receptor for high-density lipoprotein. Scavenger receptor class B, type I (SR-BI) is an integral membrane protein found in numerous cell types/tissues, including the liver and adrenal. It is best known for its role in facilitating the uptake of cholesteryl esters from high-density lipoproteins in the liver. This process drives the movement of cholesterol from peripheral tissues towards the liver for excretion. This movement of cholesterol is known as reverse cholesterol transport and is a protective mechanism against the development of atherosclerosis, which is the principal cause of heart disease and stroke. SR-BI has also been identified in the livers of non-mammalian species (turtle, goldfish, shark, chicken, frog, and skate), suggesting it emerged early in vertebrate evolutionary history. The turtle also seems to upregulate SB-RI during egg development, indicating that cholesterol efflux may be at peak levels during developmental stages.

Gene Full Name: scavenger receptor class B, member 1

Molecular Weight: 57 kDa

Gene ID: 20778

UniProt: Q61009

Pathways: Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Lipid Metabolism, SARS-CoV-2 Protein Interactome