SMAD3 antibody (Middle Region)

Catalog No. ABIN3043340

There are 4+ publications for this product available. The Rabbit Polyclonal anti-SMAD3 antibody is suitable to detect SMAD3 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for SMAD3 antibody (Middle Region) (ABIN3043340)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMAD3 Antibody

Binding Specificity: Middle Region

Purpose: Anti-Smad3 Antibody

Sequence: DHQMNHSMDA GSPNLSPNPM SPAHNNLDLQ

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse,rat sequences.

Characteristics: Anti-Smad3 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Smad3, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-SMAD3 antibody (ABIN3043340)

Cheng, Ge, Lan, Ma, Chi, Kuang, Sun, Zhao, Liu, Feng, Huang, Luo, Li, Zhang, Hu, Xu, Liu, Huo, Deng, Yang, Xi, Zhang, Siegenthaler, Chen: "Zoledronate dysregulates fatty acid metabolism in renal tubular epithelial cells to induce nephrotoxicity." in: Archives of toxicology, Vol. 92, Issue 1, pp. 469-485, (2017) (PubMed).

Xu, Ni, Huang, Meng, He, Zhang, Li: "NLRC5 Mediates IL-6 and IL-1? Secretion in LX-2 Cells and Modulated by the NF-?B/Smad3 Pathway." in: Inflammation, Vol. 38, Issue 5, pp. 1794-804, (2015) (PubMed).

Cheng, Xiao, Ainiwaer, Wang, Wu, Mao, Yang, Bao: "Molecular responses of radiation-induced liver damage in rats." in: Molecular medicine reports, Vol. 11, Issue 4, pp. 2592-600, (2015) (PubMed).

Shi, Jiang, Ma, Xie, Duan, Chen, Song, Yu, Liu, Zhu, Zheng: "Dexamethasone attenuates bleomycin-induced lung fibrosis in mice through TGF-?, Smad3 and JAK-STAT pathway." in: International journal of clinical and experimental medicine, Vol. 7, Issue 9, pp. 2645-50, (2014) (PubMed).

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background:

Background: Mothers against decapentaplegic homolog 3 also known as SMAD family member 3 or SMAD3 is a protein that in humans is encoded by the SMAD3 gene. SMAD3 is a member of the SMAD family of proteins. It is located in 15q22.33. And it mediates the signals from the transforming growth factor beta (TGF-β) super family ligands that regulate cell proliferation, differentiation and death. Based on its essential role in TGF beta signaling pathway, SMAD3 has been related with tumor growth in cancer development.

Gene Full Name: SMAD family member 3

Sequence Similarities: Belongs to the dwarfin/SMAD family.

Molecular Weight: 48 kDa

Gene ID: 4088

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy