Syndecan 1 antibody (C-Term)

Catalog No. ABIN3043430

There are 2+ publications for this product available. The Rabbit Polyclonal anti-Syndecan 1 antibody is suitable to detect Syndecan 1 in samples from Human. It has been validated for WB, FACS, ICC and IF.

Quick Overview for Syndecan 1 antibody (C-Term) (ABIN3043430)

Target: Syndecan 1 (SDC1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syndecan 1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Syndecan 1 Antibody

Binding Specificity: C-Term

Purpose: Anti-Syndecan-1/SDC1 Antibody

Sequence: ENTAVVAVEP DRRNQSPVDQ GATGASQGLL DRKE

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by six amino acids,and from the related rat sequence by four amino acids.

Characteristics: Anti-Syndecan-1/SDC1 Antibody. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Syndecan-1, different from the related mouse sequence by six amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Syndecan 1 antibody (ABIN3043430)

Yao, Li, Li, Feng, Gao: "Midkine promotes perineural invasion in human pancreatic cancer." in: World journal of gastroenterology, Vol. 20, Issue 11, pp. 3018-24, (2014) (PubMed).

Huang, Zhu, Zhang, Zhu, Liu, Zhu, Wang, Li, Yang, Dong, Liu, Chen, Zhang, Yang, Deng, Fan, Wang, Liu, Ma, Fu, Wu: "S100+ cells: a new neuro-immune cross-talkers in lymph organs." in: Scientific reports, Vol. 3, pp. 1114, (2013) (PubMed).

Target Details for Syndecan 1

Target: Syndecan 1 (SDC1)

Alternative Name: SDC1

Background:

Background: Syndecan 1 is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.

Gene Full Name: syndecan 1

Molecular Weight: 50 kDa

Gene ID: 6382

UniProt: P18827

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism