Src antibody (AA 2-368)
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- Target See all Src Antibodies
- Src (Proto-oncogene tyrosine-protein kinase Src (Src))
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Binding Specificity
- AA 2-368
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Src antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Proto-oncogene tyrosine-protein kinase Src(SRC) detection. Tested with WB, IHC-P in Human.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Proto-oncogene tyrosine-protein kinase Src(SRC) detection. Tested with WB, IHC-P in Human.
Gene Name: SRC proto-oncogene, non-receptor tyrosine kinase
Protein Name: Proto-oncogene tyrosine-protein kinase Src - Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human Src recombinant protein (Position: G2-D368). Human Src shares 97% and 99% amino acid (aa) sequence identity with mouse and rat Src, respectively.
- Isotype
- IgG
- Top Product
- Discover our top product Src Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Src is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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Slit2/Robo1 signaling promotes intestinal tumorigenesis through Src-mediated activation of the Wnt/?-catenin pathway." in: Oncotarget, Vol. 6, Issue 5, pp. 3123-35, (2015) (PubMed).
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Slit2/Robo1 signaling promotes intestinal tumorigenesis through Src-mediated activation of the Wnt/?-catenin pathway." in: Oncotarget, Vol. 6, Issue 5, pp. 3123-35, (2015) (PubMed).
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- Target
- Src (Proto-oncogene tyrosine-protein kinase Src (Src))
- Alternative Name
- SRC (Src Products)
- Synonyms
- AW259666 antibody, pp60c-src antibody, C-src1 antibody, CG7524 antibody, D-Src64B antibody, D-src antibody, DSRC64 antibody, DSrc antibody, DSrc64 antibody, DSrc64B antibody, Dm SRC1 antibody, DmHD-358 antibody, Dmel\CG7524 antibody, Dsrc antibody, Dsrc64 antibody, Dsrc64B antibody, HD-358 antibody, MRE5 antibody, SRC 64B antibody, Src antibody, Src1 antibody, Src64 antibody, c-src antibody, dSrc antibody, dsrc antibody, src antibody, src1 antibody, src64 antibody, src64B antibody, src64b antibody, ASV antibody, SRC1 antibody, c-SRC antibody, p60-Src antibody, c-Src antibody, PP60C-SCR antibody, SDR antibody, SRC proto-oncogene, non-receptor tyrosine kinase antibody, uncharacterized LOC5574802 antibody, proto-oncogene tyrosine-protein kinase src antibody, Rous sarcoma oncogene antibody, Src oncogene at 64B antibody, SRC antibody, LOC5574802 antibody, CpipJ_CPIJ010659 antibody, Src antibody, Src64B antibody
- Target Type
- Viral Protein
- Background
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Sarcoma (Scr) is a proto-oncogenic tyrosine kinase originally discovered by J. Michael Bishop and Harold E. Varmus. belongs to a family of non-receptor tyrosine kinases called Src family kinases. The human SRC protooncogene was assigned to chromosome 20. Its gene is mapped to 20q12-q13. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry. This proto-oncogene may play a role in the regulation of embryonic development and cell growth.
Synonyms: ASV antibody|Avian sarcoma virus antibody|c SRC antibody|CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody|cSrc antibody|EC 2.7.10.2 antibody|Neuronal CSRC tyrosine specific protein kinase antibody|Neuronal SRC antibody|Oncogene SRC antibody|OTTHUMP00000030931 antibody|OTTHUMP00000174476 antibody|OTTHUMP00000174477 antibody|p60 Src antibody|p60-Src antibody|p60Src antibody|pp60c src antibody|pp60c-src antibody|pp60csrc antibody|Proto oncogene tyrosine protein kinase Src antibody|Proto-oncogene c-Src antibody|Proto-oncogene tyrosine-protein kinase Src antibody|Protooncogene SRC antibody|protooncogene SRC Rous sarcoma antibody|Src antibody|SRC Oncogene antibody|SRC_HUMAN antibody|SRC1 antibody|Tyrosine kinase pp60c src antibody|Tyrosine protein kinase SRC 1 antibody|Tyrosine protein kinase SRC1 antibody|v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody|V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody|v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody|vsrc avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody - Gene ID
- 6714
- UniProt
- P12931
- Pathways
- JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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