POR antibody (C-Term)

Catalog No. ABIN3043443

The Rabbit Polyclonal anti-POR antibody (ABIN3043443) specifically detects POR in WB, IHC, ICC and FACS. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for POR antibody (C-Term) (ABIN3043443)

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-POR Antibody

Binding Specificity: C-Term

Purpose: Anti-Cytochrome P450 Reductase/POR Antibody

Sequence: RNMARDVQNT FYDIVAELGA MEHAQAVDYI KKLMTK

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse,rat sequences by five amino acids.

Characteristics: Anti-Cytochrome P450 Reductase/POR Antibody. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human POR , different from the related mouse and rat sequences by five amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Western blot, 0.1-0.5 μg/mL, Human, Mouse, RatImmunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human Immunocytochemistry, 0.5-1 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for POR

Target: POR (P450 (Cytochrome) Oxidoreductase (POR))

Alternative Name: POR

Background:

Background: POR is a membrane-boundenzyme required for electron transfer from NADPH to cytochrome P450 in the endoplasmic reticulum of theeukaryotic cell. The gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons ly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.

Gene Full Name: cytochrome p450 oxidoreductase

Molecular Weight: 77 kDa

Gene ID: 5447

UniProt: P16435

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, SARS-CoV-2 Protein Interactome