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Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (AA 175-555) Primary Antibody

PRF1 Reactivity: Human WB Host: Rabbit Polyclonal
Catalog No. ABIN3043547
$240.00
Plus shipping costs $45.00
100 μg
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  • Target
    Binding Specificity
    AA 175-555
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This Perforin 1 antibody is un-conjugated
    Application
    Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for Perforin-1(PRF1) detection. Tested with WB in Human.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Perforin-1(PRF1) detection. Tested with WB in Human.
    Gene Name: perforin 1 (pore forming protein)
    Protein Name: Perforin-1
    Purification
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human Perforin recombinant protein (Position: E175-W555). Human Perforin shares 68% amino acid (aa) sequence identity with both mouse and rat Perforin.
    Isotype
    IgG
  • Target
    Alternative Name
    PRF1 (PRF1 Antibody Abstract)
    Background
    PRF1, also known as Perforin-1, is a protein that in humans is encoded by the PRF1 gene. It is mapped to 10q22.1. PRF1 is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, PRF1 inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. PRF1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. PRF1 is thought to act by creating holes in the plasma membrane which triggers an influx of calcium and initiates membrane repair mechanisms. These repair mechanisms bring perforin and granzymes into early endosomes.

    Synonyms: Cytolysin antibody|FLH2 antibody|HPLH2 antibody|Lymphocyte pore forming protein antibody|Lymphocyte pore-forming protein antibody|MGC65093 antibody|P1 antibody|PERF_HUMAN antibody|perforin 1 (pore forming protein) antibody|Perforin 1 antibody|Perforin 1 precursor antibody|Perforin 1 preforming protein antibody|Perforin-1 antibody|PFP antibody|PGFL antibody|PIGF antibody|PIGF-2 antibody|PLGF antibody|Pore forming protein antibody|PRF 1 antibody|PRF1 antibody|SHGC-10760 antibody
    Gene ID
    5551
    UniProt
    P14222
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Perforin is approximately 0.25 ng/lane under reducing conditions.
    Notes: Tested Species: Species with positive results.
    Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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