OTX2 antibody (C-Term)

Catalog No. ABIN3043586

The Rabbit Polyclonal anti-OTX2 antibody has been validated for WB. It is suitable to detect OTX2 in samples from Human.

Quick Overview for OTX2 antibody (C-Term) (ABIN3043586)

Target: OTX2 (Orthodenticle Homeobox 2 (OTX2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OTX2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-OTX2 Antibody

Binding Specificity: AA 258-289, C-Term

Purpose: Anti-Otx2 Antibody Picoband®

Sequence: DYKDQTASWK LNFNADCLDY KDQTSSWKFQ VL

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Otx2 Antibody Picoband® (ABIN3043586). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Otx2, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Bunt J, et al. OTX2 sustains a bivalent-like state of OTX2-bound promoters in medulloblastoma by maintaining their H3K27me3 levels. Acta Neuropathol, 2013 Mar. 2. Gat-Yablonski G. Brain development is a multi-level regulated process--the case of the OTX2 gene. Pediatr Endocrinol Rev, 2011 Sep. 3. Liu Z, et al. Specific expression pattern of a novel Otx2 splicing variant during neural differentiation. Gene, 2013 Jul 1.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for OTX2

Target: OTX2 (Orthodenticle Homeobox 2 (OTX2))

Alternative Name: OTX2

Background:

Synonyms: Homeobox protein OTX2,Orthodenticle homolog 2,OTX2,

Tissue Specificity: Expressed in brain.

Background: OTX2 is also known as CPHD6 or MCOPS5. This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine.

Sequence Similarities: Belongs to the paired homeobox family. Bicoid subfamily.

Molecular Weight: 32 kDa

Gene ID: 5015

UniProt: P32243

Pathways: Dopaminergic Neurogenesis