PIM1 antibody (C-Term)

Catalog No. ABIN3043606

This Rabbit Polyclonal antibody specifically detects PIM1 in WB. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for PIM1 antibody (C-Term) (ABIN3043606)

Target: PIM1 (Pim-1 Oncogene (PIM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIM1 Antibody

Binding Specificity: AA 373-404, C-Term

Purpose: Anti-PIM1 Antibody Picoband®

Sequence: EEIQNHPWMQ DVLLPQETAE IHLHSLSPGP SK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PIM1 Antibody Picoband® (ABIN3043606). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PIM1, different from the related mouse sequence by seven amino acids and from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Amson, R., Sigaux, F., Przedborski, S., Flandrin, G., Givol, D., Telerman, A. The human protooncogene product p33pim is expressed during fetal hematopoiesis and in diverse leukemias. Proc. Nat. Acad. Sci. 86: 8857-8861, 1989. 2. Bachmann M, Möröy T (April 2005). "The serine/threonine kinase Pim-1". Int. J. Biochem. Cell Biol. 37 (4): 726-30. 3. Muraski, J. A., Rota, M., Misao, Y., Fransioli, J., Cottage, C., Gude, N., Esposito, G., Delucchi, F., Arcarese, M., Alvarez, R., Siddiqi, S., Emmanuel, G. N., and 13 others. Pim-1 regulates cardiomyocyte survival downstream of Akt. Nature Med. 13: 1467-1475, 2007. Note: Erratum: Nature Med. 14: 350 only, 2008.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-PIM1 antibody (ABIN3043606)

Wu, Lu, He, Jin: "PIM1 polymorphism and PIM1 expression as predisposing factors of esophageal squamous cell carcinoma in the Asian population." in: OncoTargets and therapy, Vol. 9, pp. 2919-25, (2016) (PubMed).

Target Details for PIM1

Target: PIM1 (Pim-1 Oncogene (PIM1))

Alternative Name: PIM1

Background:

Synonyms: Serine/threonine-protein kinase pim-1,2.7.11.1,PIM1,

Tissue Specificity: Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines. .

Background: Proto-oncogene serine/threonine-protein kinase Pim-1 is an enzyme that in humans is encoded by the PIM1 gene. It is mapped to 6p21.2. Primarily expressed in spleen, thymus, bone marrow, prostate, oral epithelial, hippocampus and fetal liver cells, Pim-1 has also been found to be highly expressed in cell cultures isolated from human tumors. Pim-1 is mainly involved in cell cycle progression, apoptosis and transcriptional activation, as well as more general signal transduction pathways. It has been found a physiologic role of the PIM1 oncogene during hematopoietic development and a deregulation of the gene in various leukemias. PIM1 also has a role in cardioprotection downstream of AKT activation.

Sequence Similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.

Molecular Weight: 44 kDa

Gene ID: 5292

UniProt: P11309

Pathways: Glycosaminoglycan Metabolic Process