JNK2 antibody (Middle Region)

Catalog No. ABIN3043612

This Rabbit Polyclonal antibody specifically detects JNK2 in WB. It exhibits reactivity toward Human.

Quick Overview for JNK2 antibody (Middle Region) (ABIN3043612)

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JNK2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-JNK2 Antibody

Binding Specificity: AA 257-288, Middle Region

Purpose: Anti-JNK2/MAPK9 Antibody Picoband®

Sequence: RNYVENRPKY PGIKFEELFP DWIFPSESER DK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-JNK2/MAPK9 Antibody (ABIN3043612). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human JNK2, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Raciti M, Lotti LV, Valia S, Pulcinelli FM, Di Renzo L. ""JNK2 is activated during ER stress and promotes cell survival"". Cell Death Dis, 2012 Nov 22. 2. Wang P, Xiong Y, Ma C, Shi T, Ma D. ""Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1"". BMB Rep, 2010 Nov.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for JNK2

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Alternative Name: MAPK9

Background:

Synonyms: Mitogen-activated protein kinase 9,MAP kinase 9,MAPK 9,2.7.11.24,JNK-55,Stress-activated protein kinase 1a,SAPK1a,Stress-activated protein kinase JNK2,c-Jun N-terminal kinase 2,MAPK9,JNK2, PRKM9, SAPK1A,

Background: JNK2 is also known as MAPK9. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. Also, this gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.

Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Molecular Weight: 48 kDa

Gene ID: 5601

UniProt: P45984

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins