CBS antibody (AA 331-551)

Catalog No. ABIN3043747

This Rabbit Polyclonal antibody specifically detects CBS in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for CBS antibody (AA 331-551) (ABIN3043747)

Target: CBS (Cystathionine-beta-Synthase (CBS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CBS Antibody

Binding Specificity: AA 331-551

Purpose: Anti-Cystathionine beta-synthase CBS Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human CBS shares 83% amino acid (aa) sequence identity with both mouse,rat CBS.

Characteristics: Anti-Cystathionine beta-synthase CBS Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CBS recombinant protein (Position: A331-K551). Human CBS shares 83% amino acid (aa) sequence identity with both mouse and rat CBS.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, HumanWestern blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CBS antibody (ABIN3043747)

Chen, Wang, Li, Jiang, Lu, Zhou: "Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats." in: Journal of diabetes research, Vol. 2016, pp. 9726503, (2016) (PubMed).

Target Details for CBS

Target: CBS (Cystathionine-beta-Synthase (CBS))

Alternative Name: CBS

Background:

Background: Cystathionine-β-synthase, also known as CBS, is an enzyme that in humans is encoded by the CBS gene. It is mapped to 21q22.3 and contains 23 exons, ranging in size from 42 to 299 bp. CBS catalyzes the first step of the transsulfuration pathway, from homocysteine to cystathionine. It uses the cofactor pyridoxal-phosphate (PLP) and can be allosterically regulated by effectors such as the ubiquitous cofactor S-adenosyl-L-methionine (adoMet). This enzyme belongs to the family of lyases, to be specific, the hydro-lyases, which cleave carbon-oxygen bonds. CBS is a multidomain enzyme composed of an N-terminal enzymatic domain and two CBS domains. The CBS gene is the most common locus for mutations associated with homocystinuria.

Gene Full Name: cystathionine beta-synthase like

Sequence Similarities: Belongs to the cysteine synthase/cystathionine beta- synthase family.

Molecular Weight: 65 kDa

Gene ID: 875

UniProt: P35520