Caspase 9 antibody (AA 3-228)

Catalog No. ABIN3043748

The Rabbit Polyclonal anti-Caspase 9 antibody has been validated for WB. It is suitable to detect Caspase 9 in samples from Human. There are 17+ publications available.

Quick Overview for Caspase 9 antibody (AA 3-228) (ABIN3043748)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 9 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 9 Antibody

Binding Specificity: AA 3-228

Purpose: Anti-Caspase-9/CASP9 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Caspase-9/CASP9 Antibody Picoband® (ABIN3043748). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Caspase-9 recombinant protein (Position: E3-D228). Human Caspase-9 shares 63% amino acid (aa) sequence identity with mouse Caspase-9.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Cingeetham A, et al. Association of caspase9 promoter polymorphisms with the susceptibility of AML in south Indian subjects. Tumour Biol, 2014 Sep. 2. Xu D, et al. Apoptotic block in colon cancer cells may be rectified by lentivirus mediated overexpression of caspase-9. Acta Gastroenterol Belg, 2013 Dec.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Caspase 9 antibody (ABIN3043748)

Bai, Yang, Luo: "Effects of 5-hydroxy-4'-nitro-7-propionyloxy-genistein on inhibiting proliferation and invasion via activating reactive oxygen species in human ovarian cancer A2780/DDP cells." in: Oncology letters, Vol. 15, Issue 4, pp. 5227-5235, (2018) (PubMed).

Ji, Geng, Zhou, Wei, Chen: "Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells." in: Neural regeneration research, Vol. 11, Issue 12, pp. 1962-1968, (2016) (PubMed).

Ding, Wang, Zhao, Sun, Zhai: "Protective Effects of Baicalin on A?????-Induced Learning and Memory Deficit, Oxidative Stress, and Apoptosis in Rat." in: Cellular and molecular neurobiology, Vol. 35, Issue 5, pp. 623-32, (2015) (PubMed).

Yang, Li, Liu, Shi, Zhang: "Inhibitory effect of tetramethylpyrazine preconditioning on overload training-induced myocardial apoptosis in rats." in: Chinese journal of integrative medicine, Vol. 21, Issue 6, pp. 423-30, (2015) (PubMed).

Chen, Peng, Rowat, Gao, Zhang, Wang, Zhang, Wang, Qu: "The effect of concentration and duration of normobaric oxygen in reducing caspase-3 and -9 expression in a rat-model of focal cerebral ischaemia." in: Brain research, Vol. 1618, pp. 205-11, (2015) (PubMed).

Huang, Lin, Su, Ye: "Forsythiaside protects against hydrogen peroxide-induced oxidative stress and apoptosis in PC12 cell." in: Neurochemical research, Vol. 40, Issue 1, pp. 27-35, (2015) (PubMed).

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

Yan, Liang, Zhang, Liu, Bu: "Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL-RVG) in vitro." in: Molecular medicine reports, Vol. 11, Issue 1, pp. 317-26, (2014) (PubMed).

Wei, Tian, Yan, Shao, Xie: "Protective effects of phillyrin on H2O 2-induced oxidative stress and apoptosis in PC12 cells." in: Cellular and molecular neurobiology, Vol. 34, Issue 8, pp. 1165-73, (2014) (PubMed).

Tian, Wei, Li, Wang, Zhang, Xie: "Pathway of programmed cell death and oxidative stress induced by ?-hydroxybutyrate in dairy cow abomasum smooth muscle cells and in mouse gastric smooth muscle." in: PLoS ONE, Vol. 9, Issue 5, pp. e96775, (2014) (PubMed).

Song, Wang, Chen, Zhang, Wang, Liu, Gao, Yan: "Toxic effects of sodium fluoride on cell proliferation and apoptosis of Leydig cells from young mice." in: Journal of physiology and biochemistry, Vol. 70, Issue 3, pp. 761-8, (2014) (PubMed).

Dai, Li, Tang, Li, Xiao: "Colistin-induced nephrotoxicity in mice involves the mitochondrial, death receptor, and endoplasmic reticulum pathways." in: Antimicrobial agents and chemotherapy, Vol. 58, Issue 7, pp. 4075-85, (2014) (PubMed).

Yuan, Sun, Ou, Wang, Zhang, Deng, Wu, Zhang: "Apoptosis is an obstacle to the differentiation of adipose-derived stromal cells into astrocytes." in: Neural regeneration research, Vol. 9, Issue 8, pp. 837-44, (2014) (PubMed).

Liu, Xiong, Liu, Miao, Lin, Wen: "In vivo and in vitro evaluation of the cytotoxic effects of Photosan-loaded hollow silica nanoparticles on liver cancer." in: Nanoscale research letters, Vol. 9, Issue 1, pp. 319, (2014) (PubMed).

Chang, Liu, Fang, He, Wang, Zhu, Jiang: "Effects of matrine on the proliferation of HT29 human colon cancer cells and its antitumor mechanism." in: Oncology letters, Vol. 6, Issue 3, pp. 699-704, (2013) (PubMed).

Ge, Yu, Petitte, Zhang: "Epidermal growth factor-induced proliferation of chicken primordial germ cells: involvement of calcium/protein kinase C and NFKB1." in: Biology of reproduction, Vol. 80, Issue 3, pp. 528-36, (2009) (PubMed).

Li, Chu, Gao, Li: "Apoptotic mechanism of MCF-7 breast cells in vivo and in vitro induced by photodynamic therapy with C-phycocyanin." in: Acta biochimica et biophysica Sinica, Vol. 42, Issue 1, pp. 80-9, (2009) (PubMed).

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: CASP9

Background:

Synonyms: Caspase-9,CASP-9,3.4.22.62,Apoptotic protease Mch-6,Apoptotic protease-activating factor 3,APAF-3,ICE-like apoptotic protease 6,ICE-LAP6,Caspase-9 subunit p35,Caspase-9 subunit p10,CASP9,MCH6,

Tissue Specificity: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. .

Background: CASP9 is also known as MCH6 or APAF3. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1, this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Sequence Similarities: Belongs to the peptidase C14A family.

Molecular Weight: 35 kDa

Gene ID: 842

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity