Angiotensin I Converting Enzyme 1 antibody (AA 651-864)

Catalog No. ABIN3043777

This Rabbit Polyclonal antibody specifically detects Angiotensin I Converting Enzyme 1 in WB, IHC, ICC, IHC (fro) and IF. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for Angiotensin I Converting Enzyme 1 antibody (AA 651-864) (ABIN3043777)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiotensin I Converting Enzyme 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Product Details anti-Angiotensin I Converting Enzyme 1 Antibody

Binding Specificity: AA 651-864

Purpose: Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband® (ABIN3043777). Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ACE recombinant protein (Position: K651-Y864). Human ACE shares 73% and 76% amino acid (aa) sequences identity with mouse and rat ACE, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human1. Kierszenbaum, Abraham L. (2007). Histology and cell biology: an introduction to pathology. Mosby Elsevier 2. Jeunemaitre, X., Lifton, R. P., Hunt, S. C., Williams, R. R., Lalouel, J.-M. Absence of linkage between the angiotensin converting enzyme locus and human essential hypertension. Nature Genet. 1: 72-75, 1992. 3. Mattei, M.-G., Hubert, C., Alhenc-Gelas, F., Roeckel, N., Corvol, P., Soubrier, F. Angiotensin-I converting enzyme gene is on chromosome 17. (Abstract) Cytogenet. Cell Genet. 51: 1041, 1989.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Angiotensin I Converting Enzyme 1 antibody (ABIN3043777)

Yan, Shen: "Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 3965-3973, (2018) (PubMed).

Target Details for Angiotensin I Converting Enzyme 1

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Alternative Name: ACE

Background:

Synonyms: Angiotensin-converting enzyme,ACE,3.2.1.-,3.4.15.1,Dipeptidyl carboxypeptidase I,Kininase II,CD143,Angiotensin-converting enzyme, soluble form,ACE,DCP, DCP1,

Tissue Specificity: Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. .

Background: Angiotensin-converting enzyme (ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), which mediates extracellular volume (i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.

Sequence Similarities: Belongs to the peptidase M2 family.

Molecular Weight: 180 kDa

Gene ID: 1636

UniProt: P12821

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration