Aquaporin 4 antibody (C-Term)

Catalog No. ABIN3043789

This Rabbit Polyclonal antibody specifically detects Aquaporin 4 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 5+ publications.

Quick Overview for Aquaporin 4 antibody (C-Term) (ABIN3043789)

Target: Aquaporin 4 (AQP4)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Aquaporin 4 Antibody

Binding Specificity: AA 295-323, C-Term

Purpose: Anti-Aquaporin 4/AQP4 Antibody Picoband®

Sequence: KPGVVHVIDV DRGEEKKGKD QSGEVLSSV

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Aquaporin 4/AQP4 Antibody Picoband® (ABIN3043789). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat
1. Amiry-Moghaddam, M., Otsuka, T., Hurn, P. D., Traystman, R. J., Haug, F.-M., Froehner, S. C., Adams, M. E., Neely, J. D., Agre, P., Ottersen, O. P., Bhardwaj, A. : An alpha-syntrophin-dependent pool of AQP4 in astroglial end-feet confers biional water flow between blood and brain. Proc. Nat. Acad. Sci. 100: 2106-2111, 2003. 2. Agre P, Nielsen S (1996). "The aquaporin family of water channels in kidney". Nephrologie 17 (7): 409-15. 3. Lennon, V. A., Kryzer, T. J., Pittock, S. J., Verkman, A. S., Hinson, S. R. : IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel. J. Exp. Med. 202: 473-477, 2005. 4. Lu, M., Lee, M. D., Smith, B. L., Jung, J. S., Agre, P., Verdijk, M. A. J., Merkx, G., Rijss, J. P. L., Deen, P. M. T. : The human AQP4 gene: definition of the locus encoding two water channel polypeptides in brain. Proc. Nat. Acad. Sci. 93: 10908-10912, 1996. 5. Nagelhus EA, Mathiisen TM, Ottersen OP (2004). "Aquaporin-4 in the central nervous system: cellular and subcellular distribution and coexpression with KIR4.1". Neuroscience 129 (4): 905-13.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Aquaporin 4 antibody (ABIN3043789)

Han, Huang, Liu, Sha, Hu, Liu: "Changes in the Expression of AQP4 and AQP9 in the Hippocampus Following Eclampsia-Like Seizure." in: International journal of molecular sciences, Vol. 19, Issue 1, (2018) (PubMed).

Anzabi, Ardalan, Iversen, Rafati, Hansen, Østergaard: "Hippocampal Atrophy Following Subarachnoid Hemorrhage Correlates with Disruption of Astrocyte Morphology and Capillary Coverage by AQP4." in: Frontiers in cellular neuroscience, Vol. 12, pp. 19, (2018) (PubMed).

Wang, Bu, Zhang, Chen, Zhang, Bao: "Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema." in: Molecular medicine reports, Vol. 12, Issue 4, pp. 5625-32, (2016) (PubMed).

Zhang, Chen, Dang, Liu, Ito, Sun: "Neuroprotective effects of total steroid saponins on cerebral ischemia injuries in an animal model of focal ischemia/reperfusion." in: Planta medica, Vol. 80, Issue 8-9, pp. 637-44, (2015) (PubMed).

Chen, Huang, Ruan, He, Li: "Ulinastatin attenuates cerebral ischemia-reperfusion injury in rats." in: International journal of clinical and experimental medicine, Vol. 7, Issue 5, pp. 1483-9, (2014) (PubMed).

Target Details for Aquaporin 4

Target: Aquaporin 4 (AQP4)

Alternative Name: AQP4

Background:

Synonyms: Aquaporin-4,AQP-4,Mercurial-insensitive water channel,MIWC,WCH4,AQP4,

Tissue Specificity: Brain - muscle >> heart, kidney, lung, and trachea.

Background: Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1. Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0, the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by insult to the central nervous system. And AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.

Sequence Similarities: Belongs to the MIP/aquaporin (TC 1.A.8) family.

Molecular Weight: 37 kDa

Gene ID: 361

UniProt: P55087

Pathways: Sensory Perception of Sound