ATP2A1/SERCA1 antibody (N-Term)

Catalog No. ABIN3043792

This Rabbit Polyclonal antibody specifically detects ATP2A1/SERCA1 in WB, IHC and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for ATP2A1/SERCA1 antibody (N-Term) (ABIN3043792)

Target: ATP2A1/SERCA1 (ATP2A1) (ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1 (ATP2A1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP2A1/SERCA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-ATP2A1/SERCA1 Antibody

Binding Specificity: AA 1-32, N-Term

Purpose: Anti-SERCA1 ATPase/ATP2A1 Antibody

Sequence: MEAAHAKTTE ECLAYFGVSE TTGLTPDQVK RN

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SERCA1 ATPase/ATP2A1 Antibody (ABIN3043792). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SERCA1 ATPase, different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ATP2A1/SERCA1

Target: ATP2A1/SERCA1 (ATP2A1) (ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1 (ATP2A1))

Alternative Name: ATP2A1

Background:

Synonyms: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1,SERCA1,SR Ca (2+)-ATPase 1,3.6.3.8,Calcium pump 1,Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform,Endoplasmic reticulum class 1/2 Ca (2+) ATPase,ATP2A1,

Tissue Specificity: Skeletal muscle, fast twitch muscle (type II) fibers.

Background: SERCA1, also called ATP2A1, is an enzyme that in humans is encoded by the ATP2A1 gene. This gene encodes one of the SERCA Ca (2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. The SERCA1 gene is mapped to 16p11.2. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. It has been determined that the human SERCA1 gene is 26 kb long and contains 23 exons, of which can be alternatively spliced. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise.

Molecular Weight: 110 kDa

Gene ID: 487

UniProt: O14983

Pathways: Ribonucleoside Biosynthetic Process