COMT antibody (AA 52-271)

Catalog No. ABIN3043815

This anti-COMT antibody is a Rabbit Polyclonal antibody detecting COMT in WB, IHC and ICC. Suitable for Human, Rat and Mouse.

Quick Overview for COMT antibody (AA 52-271) (ABIN3043815)

Target: COMT (Catechol-O-Methyltransferase (COMT))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-COMT Antibody

Binding Specificity: AA 52-271

Purpose: Anti-COMT Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-COMT Antibody Picoband® (ABIN3043815). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human COMT recombinant protein (Position: G52-P271). Human COMT shares 81.9% and 81% amino acid (aa) sequence identity with mouse and rat COMT, respectively.

Isotype: IgG

Application Details

Application Notes: Immunocytochemistry , 0.5-1 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Western blot, 0.1-0.5 μg/mL
1. Baker, K., Baldeweg, T., Sivagnanasundaram, S., Scambler, P., Skuse, D. COMT val108/158met modifies mismatch negativity and cognitive function in 22q11 deletion syndrome. Biol. Psychiat. 58: 23-31, 2005. 2. Lee, S.-G., Joo, Y., Kim, B., Chung, S., Kim, H.-L., Lee, I., Choi, B., Kim, C., Song, K.Association of Ala72Ser polymorphism with COMT enzyme activity and the risk of schizophrenia in Koreans. Hum. Genet. 116: 319-328, 2005.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for COMT

Target: COMT (Catechol-O-Methyltransferase (COMT))

Alternative Name: COMT

Background:

Synonyms: Catechol O-methyltransferase,2.1.1.6,COMT,

Tissue Specificity: Brain, liver, placenta, lymphocytes and erythrocytes.

Background: Catechol O-methyltransferase, also called COMT, is one of the major mammalian enzymes involved in the metabolic degradation of catecholamines. This gene is mapped to 22q11.21. Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini.

Sequence Similarities: Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O- methyltransferase family.

Molecular Weight: 24 kDa, 28 kDa

Gene ID: 1312

UniProt: P21964

Pathways: Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome