GRIA2 antibody (AA 25-360)

Catalog No. ABIN3043838

The Rabbit Polyclonal anti-GRIA2 antibody has been validated for WB, IHC, ICC, FACS, IF and IHC (fro). It is suitable to detect GRIA2 in samples from Human, Rat and Mouse. There are 2+ publications available.

Quick Overview for GRIA2 antibody (AA 25-360) (ABIN3043838)

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GRIA2 Antibody

Binding Specificity: AA 25-360

Purpose: Anti-Ionotropic Glutamate receptor 2/GRIA2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Ionotropic Glutamate receptor 2/GRIA2 Antibody Picoband® (ABIN3043838). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GRIA2 recombinant protein (Position: N25-I360). Human GRIA2 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat
Immunocytochemistry, 0.5-1 μg/mL, Human
Immunofluorescence, 2 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Armstrong, N., Gouaux, E. Mechanisms for activation and antagonism of an AMPA-sensitive glutamate receptor: crystal structures of the GluR2 ligand binding core. Neuron 28: 165-181, 2000. 2. Biou, V., Bhattacharyya, S., Malenka, R. C. Endocytosis and recycling of AMPA receptors lacking GluR2/3. Proc. Nat. Acad. Sci. 105: 1038-1043, 2008. 3. Mameli, M., Balland, B., Lujan, R., Luscher, C. Rapid synthesis and synaptic insertion of GluR2 for mGluR-LTD in the ventral tegmental area. Science 317: 530-533, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-GRIA2 antibody (ABIN3043838)

Liu, Mei, Li, Roboti, Pang, Ying, Gao, Lowe, Bao: "Loss of the golgin GM130 causes Golgi disruption, Purkinje neuron loss, and ataxia in mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 2, pp. 346-351, (2018) (PubMed).

Dai, Li, Zhong, Chen, Cheng, Liu, Li: "The paracrine effect of cobalt chloride on BMSCs during cognitive function rescue in the HIBD rat." in: Behavioural brain research, Vol. 332, pp. 99-109, (2017) (PubMed).

Target Details for GRIA2

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Alternative Name: GRIA2

Background:

Synonyms: Glutamate receptor 2,GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic, AMPA 2,GluA2,GRIA2,GLUR2,

Tissue Specificity: Widely expressed in brain.

Background: Glutamate receptor 2, also known as GLUR2, is a protein that in humans is encoded by the GRIA2 gene. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. GLUR2's cytogenetic location is 4q32.1. The crystal structures of the GLUR2 ligand-binding core in the apo state and in the presence of the antagonist DNQX, the partial agonist kainate, and the full agonists AMPA and glutamate.GLUR2 plays a major role in depression at synapses in which glutamate remains in the synaptic cleft for prolonged periods of time during normal operation of the synapse. The overexpression of GLUR2 increases dendritic spine size and density in hippocampal neurons, and more remarkably, induces spine formation in GABA-releasing interneurons that normally lack spines.

Molecular Weight: 110 kDa

Gene ID: 14800

UniProt: P42262

Pathways: PI3K-Akt Signaling