HIF1A antibody (C-Term)

Catalog No. ABIN3043841

The Rabbit Polyclonal anti-HIF1A antibody has been validated for WB and IHC. It is suitable to detect HIF1A in samples from Human, Mouse and Rat. There are 33+ publications available.

Quick Overview for HIF1A antibody (C-Term) (ABIN3043841)

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HIF1A Antibody

Binding Specificity: AA 703-732, C-Term

Purpose: Anti-HIF-1-alpha/HIF1A Antibody Picoband®

Sequence: EEELNPKILA LQNAQRKRKM EHDGSLFQAV

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HIF-1-alpha/HIF1A Antibody Picoband® (ABIN3043841). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human HIF-1-alpha, different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse
1. Sutter, C. H., Laughner, E., Semenza, G. L. : Hypoxia-inducible factor 1-alpha protein expression is controlled by oxygen-regulated ubiquitination that is disrupted by deletions and missense mutations. Proc. Nat. Acad. Sci. 97: 4748-4753, 2000. 2. Elson, D. A., Thurston, G., Huang, L. E., Ginzinger, D. G., McDonald, D. M., Johnson, R. S., Arbeit, J. M. : Induction of hypervascularity without leakage or inflammation in transgenic mice overexpressing hypoxia-inducible factor-1-alpha. Genes Dev. 15: 2520-2532, 2001. 3. Koshiji, M., To, K. K.-W., Hammer, S., Kumamoto, K., Harris, A. L., Modrich, P., Huang, L. E. : HIF-1-alpha induces genetic instability by transcriptionally downregulating MutS-alpha expression. Molec. Cell 17: 793-803, 2005. 4. Ivan, M., Kondo, K., Yang, H., Kim, W., Valiando, J., Ohh, M., Salic, A., Asara, J. M., Lane, W. S., Kaelin, W. G., Jr. : HIF-alpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O(2) sensing. Science 292: 464-468, 2001.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-HIF1A antibody (ABIN3043841)

Wang, Yuan, Wang, Yang, Chen, Liu, Song, Feng, Tan, Jia: "Anti-inflammatory Effects of Phyllanthus emblica L on Benzopyrene-Induced Precancerous Lung Lesion by Regulating the IL-1β/miR-101/Lin28B Signaling Pathway." in: Integrative cancer therapies, Vol. 16, Issue 4, pp. 505-515, (2018) (PubMed).

Qin, Ke, Zhou, Wang, Liang, Wang, Yang, Gao, Ye, Kumar, Wang: "Metastasis-Associated Protein 1 Deficiency Results in Compromised Pulmonary Alveolar Capillary Angiogenesis in Mice." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 3932-3941, (2018) (PubMed).

Yang, Chen, Wang, Xian, Zhang, Zhu, Pan, Cheng: "TLR4 promotes the expression of HIF-1α by triggering reactive oxygen species in cervical cancer cells in vitro-implications for therapeutic intervention." in: Molecular medicine reports, Vol. 17, Issue 2, pp. 2229-2238, (2018) (PubMed).

Guo, Mu, Yu, Li, Chen, Wei, Bi, Yu, Liang: "Comparison of the expression of TGF-β1, E-cadherin, N-cadherin, TP53, RB1CC1 and HIF-1α in oral squamous cell carcinoma and lymph node metastases of humans and mice." in: Oncology letters, Vol. 15, Issue 2, pp. 1639-1645, (2018) (PubMed).

Gao, Chen, Gao, Wang, Sun, Yang: "CD47 deficiency in tumor stroma promotes tumor progression by enhancing angiogenesis." in: Oncotarget, Vol. 8, Issue 14, pp. 22406-22413, (2017) (PubMed).

Wan, Wu: "Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways." in: Journal of experimental & clinical cancer research : CR, Vol. 35, Issue 1, pp. 119, (2017) (PubMed).

Li, Shi, Huang, Wang, Yu, Guo, Ren: "Suppression of the expression of hypoxia-inducible factor-1α by RNA interference alleviates hypoxia-induced pulmonary hypertension in adult rats." in: International journal of molecular medicine, Vol. 38, Issue 6, pp. 1786-1794, (2017) (PubMed).

Liu, Murray-Stewart, Casero, Kagiampakis, Jin, Zhang, Wang, Che, Tong, Ke, Jiang, Wang, Wan: "Targeting hexokinase 2 inhibition promotes radiosensitization in HPV16 E7-induced cervical cancer and suppresses tumor growth." in: International journal of oncology, Vol. 50, Issue 6, pp. 2011-2023, (2017) (PubMed).

Ding, Chen, Xu, Qin: "Effect of Hyperbaric Oxygen on the Growth of Intracranial Glioma in Rats." in: Chinese medical journal, Vol. 128, Issue 23, pp. 3197-203, (2016) (PubMed).

Wan, Wu, Zhang: "Local recurrence of small cell lung cancer following radiofrequency ablation is induced by HIF-1α expression in the transition zone." in: Oncology reports, Vol. 35, Issue 3, pp. 1297-308, (2016) (PubMed).

Wang, Fang, Tan, Zhang, Ma: "Hypoxic preconditioning increases the protective effect of bone marrow mesenchymal stem cells on spinal cord ischemia/reperfusion injury." in: Molecular medicine reports, Vol. 13, Issue 3, pp. 1953-60, (2016) (PubMed).

Wu, Ye, Deng, Wu, Li, Zhang: "Upregulation of metastasis-associated gene 2 promotes cell proliferation and invasion in nasopharyngeal carcinoma." in: OncoTargets and therapy, Vol. 9, pp. 1647-56, (2016) (PubMed).

Xu, Dong: "Role of hypoxia-inducible factor-1α in pathogenesis and disease evaluation of ulcerative colitis." in: Experimental and therapeutic medicine, Vol. 11, Issue 4, pp. 1330-1334, (2016) (PubMed).

Ren, Wang, Xu, Dai: "Effect of rapamycin on endometriosis in mice." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 101-106, (2016) (PubMed).

Sun, He, Geng: "Neuroprotective mechanism of HIF-1α overexpression in the early stage of acute cerebral infarction in rats." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 391-395, (2016) (PubMed).

Dai, Sun, Yao, Chen, Yu, Long: "Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL-6/HIF-1α signaling pathway." in: FEBS open bio, Vol. 6, Issue 7, pp. 720-8, (2016) (PubMed).

Wang, Jing, Shan, Chen, Guo, Cao, Mu, Peng, Zhou, Li: "Semaphorin 4D and hypoxia-inducible factor-1α overexpression is related to prognosis in colorectal carcinoma." in: World journal of gastroenterology, Vol. 21, Issue 7, pp. 2191-8, (2015) (PubMed).

Wang, Wu, Guo, Zhang, Zhang, Zhan: "Increased hypoxia-inducible factor 1alpha expression in rat brain tissues in response to aging." in: Neural regeneration research, Vol. 7, Issue 10, pp. 778-82, (2015) (PubMed).

Wu, Wang, Zhang, Zhang, Zhang, Zhang: "Puerarin decreases hypoxia inducible factor-1 alpha in the hippocampus of vascular dementia rats." in: Neural regeneration research, Vol. 7, Issue 6, pp. 421-5, (2015) (PubMed).

Feng, Tao, Dawei, Xuliang, Xiaodong: "HIF-1? expression correlates with cellular apoptosis, angiogenesis and clinical prognosis in rectal carcinoma." in: Pathology oncology research : POR, Vol. 20, Issue 3, pp. 603-10, (2014) (PubMed).

Target Details for HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name: HIF1A

Background:

Synonyms: Hypoxia-inducible factor 1-alpha,HIF-1-alpha,HIF1-alpha,ARNT-interacting protein,Basic-helix-loop-helix-PAS protein MOP1,Class E basic helix-loop-helix protein 78,bHLHe78,Member of PAS protein 1,PAS domain-containing protein 8,HIF1A,BHLHE78, MOP1, PASD8,

Tissue Specificity: Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland. .

Background: HIF-1α (Hypoxia-inducible factor 1α, HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1α transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1alpha is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF-1α regulates the survival and function in the inflammatory microenvironment ly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.

Sequence Similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

Molecular Weight: 115-120 kDa

Gene ID: 3091

UniProt: Q16665

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect