MMP7 antibody (AA 1-267)

Catalog No. ABIN3043880

There are 8+ publications for this product available. The Rabbit Polyclonal anti-MMP7 antibody is suitable to detect MMP7 in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for MMP7 antibody (AA 1-267) (ABIN3043880)

Target: MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MMP7 Antibody

Binding Specificity: AA 1-267

Purpose: Anti-MMP7 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human MMP7 shares 71% amino acid (aa) sequence identity with both mouse,rat MMP7.

Characteristics: Anti-MMP7 Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MMP7 recombinant protein (Position: M1-K267). Human MMP7 shares 71% amino acid (aa) sequence identity with both mouse and rat MMP7.

Isotype: IgG

Application Details

Application Notes: ELISA, 0.1-0.5 μg/mL, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, HumanWestern blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-MMP7 antibody (ABIN3043880)

Li, Xiao, Li, Li, Zeng, Liu, Liang, Li, Chu, Yang: "Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1715-1722, (2018) (PubMed).

Jia, Jiang, Liu, Wang, Zhu, Zhu, Liu, Zhong, Xie, Huang, Jia, Li, Liu, Zuo, Cheng, Dai, Ren: "Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells." in: International journal of oncology, Vol. 52, Issue 6, pp. 1787-1800, (2018) (PubMed).

Bai, Luo: "5-Hydroxy-4'-Nitro-7-Propionyloxy-Genistein Inhibited Invasion and Metastasis via Inactivating Wnt/b-Catenin Signal Pathway in Human Endometrial Carcinoma Ji Endometrial Cells." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 3230-3243, (2018) (PubMed).

Xu, Xu, Li, Wang, Fu, Jia, Wang, Zhu, Lu, Yao: "Growth differentiation factor 15 induces growth and metastasis of human liver cancer stem-like cells via AKT/GSK-3β/β-catenin signaling." in: Oncotarget, Vol. 8, Issue 10, pp. 16972-16987, (2017) (PubMed).

Gao, Tang, He, Liu, Mao, Ji, Lin, Wu: "Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats." in: Frontiers in pharmacology, Vol. 6, pp. 215, (2015) (PubMed).

Yang, Gao, Rao, Zhang, Ouyang, Yang: "Antisense oligonucleotide targeting matrix metalloproteinase-7 (MMP-7) changes the ultrastructure of human A549 lung adenocarcinoma cells." in: Ultrastructural pathology, Vol. 35, Issue 6, pp. 256-9, (2011) (PubMed).

Yang, Shi, Li, Yi: "Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line." in: Oncology reports, Vol. 25, Issue 1, pp. 259-65, (2010) (PubMed).

Pan, Zhang, Xu, Meng, Zhang, Xue, Han, Li: "Beta-catenin signaling involves HGF-enhanced HepG2 scattering through activating MMP-7 transcription." in: Histochemistry and cell biology, Vol. 134, Issue 3, pp. 285-95, (2010) (PubMed).

Target Details for MMP7

Target: MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Alternative Name: MMP7

Background:

Background: Matrix metalloproteinase-7 (MMP-7) previously called putative metalloproteinase I (PUMP1) or matrilysin. The MMP-7 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The MMP-7 protein has 267 amino acids and is significantly shorter than stromelysin or collagenase (477 and 469 amino acids, respectively). Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. MMP-7, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, MMP-7 mRNA is regulated in a specific and distinct manner in normal and malignant tissues. MMP-7 has been shown to correlate with nodal or distant metastasis in colorectal carcinomas, however, its implication in early invasive colorectal carcinomas has not been determined. MMP-7 is also a mediator of pulmonary fibrosis and a potential therapeutic target. The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28KDa. The detected MMP-7 includes zymogen and active enzyme.

Gene Full Name: matrix metallopeptidase 7

Sequence Similarities: Belongs to the peptidase M10A family.

Molecular Weight: 30 kDa

Gene ID: 4316

UniProt: P09237

Pathways: Production of Molecular Mediator of Immune Response