PAK7 antibody (N-Term)

Catalog No. ABIN3043895

The Rabbit Polyclonal anti-PAK7 antibody has been validated for WB and IHC. It is suitable to detect PAK7 in samples from Human, Mouse and Rat.

Quick Overview for PAK7 antibody (N-Term) (ABIN3043895)

Target: PAK7 (P21 Protein (Cdc42/Rac)-Activated Kinase 7 (PAK7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PAK7 Antibody

Binding Specificity: AA 26-55, N-Term

Purpose: Anti-PAK5/PAK7 Antibody Picoband®

Sequence: DPQEQKFTGL PQQWHSLLAD TANRPKPMVD

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PAK5/PAK7 Antibody Picoband® (ABIN3043895). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PAK5, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: PAK7 p21(CDKN1A)-activated kinase 7". 2. Dan C, Nath N, Liberto M, Minden A (Dec 2001). "PAK5, a new brain-specific kinase, promotes neurite outgrowth in N1E-115 cells". Mol Cell Biol 22 (2): 567-77. 3. Nagase T, Ishikawa K, Kikuno R, Hirosawa M, Nomura N, Ohara O (Jan 2000). "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res 6 (5): 337-45.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PAK7

Target: PAK7 (P21 Protein (Cdc42/Rac)-Activated Kinase 7 (PAK7))

Alternative Name: PAK5

Background:

Synonyms: Serine/threonine-protein kinase PAK 7,2.7.11.1,p21-activated kinase 5,PAK-5,p21-activated kinase 7,PAK-7,PAK7,KIAA1264, PAK5,

Tissue Specificity: Predominantly expressed in brain.

Background: Serine/threonine-protein kinase PAK 7, also known as PAK5, is an enzyme that in humans is encoded by the PAK7 gene. The protein encoded by this gene is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. And this kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. In addition, this kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described.

Molecular Weight: 81 kDa

Gene ID: 57144

UniProt: Q9P286