PGRMC1 antibody (Middle Region)

Catalog No. ABIN3043899

The Rabbit Polyclonal anti-PGRMC1 antibody (ABIN3043899) specifically detects PGRMC1 in WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PGRMC1 antibody (Middle Region) (ABIN3043899)

Target: PGRMC1 (Progesterone Receptor Membrane Component 1 (PGRMC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGRMC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PGRMC1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-PGRMC1 Antibody

Sequence: RLKRRDFTPA ELRRFDGVQD PRILMAINGK VFDVTK

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse sequence,and different from the related rat sequence by two amino acids.

Characteristics: Anti-PGRMC1 Antibody. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PGRMC1, identical to the related mouse sequence, and different from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PGRMC1

Target: PGRMC1 (Progesterone Receptor Membrane Component 1 (PGRMC1))

Alternative Name: PGRMC1

Background:

Background: Progesterone receptor membrane component 1 (PGRMC1) is a protein which co-purifies with progesterone binding proteins in the liver and ovary. In humans, the PGRMC1 protein is encoded by the PGRMC1 gene. The Sigma-2 receptor was recently identified as potentially being the same as PGRMC1. The sole biochemical function of PGRMC1 is heme-binding. PGRMC1 shares key structural motifs with cytochrome b5. It binds and activates P450 proteins, which are important in drug, hormone and lipid metabolism. Also, PGRMC1 binds to PAIR-BP1 (plasminogen activator inhibitor RNA-binding protein-1). However, its expression outside of the reproductive tract and in males suggests multiple functions for the protein. These may include binding to Insig (insulin-induced gene), which regulates cholesterol synthesis.

Gene Full Name: progesterone receptor membrane component 1

Molecular Weight: 26 kDa

Gene ID: 10857

UniProt: O00264