TLR7 antibody (C-Term)

Catalog No. ABIN3043948

This Rabbit Polyclonal antibody specifically detects TLR7 in WB, IHC, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for TLR7 antibody (C-Term) (ABIN3043948)

Target: TLR7 (Toll-Like Receptor 7 (TLR7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-TLR7 Antibody

Binding Specificity: C-Term

Purpose: Anti-TLR7 Antibody

Sequence: QAHPYFWQCL KNALATDNHV AYSQVFKE

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by two amino acids.

Characteristics: Anti-TLR7 Antibody. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TLR7, different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, - Immunocytochemistry , 0.5-1 μg/mL, Human, - Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-TLR7 antibody (ABIN3043948)

Esancy, Condon, Feng, Kimball, Curtright, Dhaka: "A zebrafish and mouse model for selective pruritus via direct activation of TRPA1." in: eLife, Vol. 7, (2018) (PubMed).

Target Details for TLR7

Target: TLR7 (Toll-Like Receptor 7 (TLR7))

Alternative Name: TLR7

Background:

Background: TLR7 (Toll-like receptor 7) is protein that in humans is encoded by the TLR7 gene. Orthologs are found in mammals and birds. TLR7 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on human chromosome X. TLR7 recognises single stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages.

Gene Full Name: toll like receptor 7

Sequence Similarities: Belongs to the Toll-like receptor family.

Molecular Weight: 121 kDa

Gene ID: 51284

UniProt: Q9NYK1

Pathways: TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades