TNFRSF1B antibody (C-Term)

Catalog No. ABIN3043949

The Rabbit Polyclonal anti-TNFRSF1B antibody has been validated for WB and IHC. It is suitable to detect TNFRSF1B in samples from Mouse and Rat. There are 2+ publications available.

Quick Overview for TNFRSF1B antibody (C-Term) (ABIN3043949)

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFRSF1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TNFRSF1B Antibody

Binding Specificity: AA 296-324, C-Term

Purpose: Anti-TNF Receptor II/TNFRSF1B Antibody Picoband®

Sequence: QRDAKVPHVP DEKSQDAVGL EQQHLLTTA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-TNF Receptor II/TNFRSF1B Antibody Picoband® (ABIN3043949). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse TNF Receptor II, different from the related human sequence by eleven amino acids, and from the related rat sequence by six amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Mouse, Rat
1. Milatovich, A., Song, K., Heller, R. A., Francke, U. : Tumor necrosis factor receptor genes, TNFR1 and TNFR2, on human chromosomes 12 and 1. Somat. Cell Molec. Genet. 17: 519-523, 1991. 2. Vielhauer, V., Stavrakis, G., Mayadas, T. N. : Renal cell-expressed TNF receptor 2, not receptor 1, is essential for the development of glomerulonephritis. J. Clin. Invest. 115: 1199-1209, 2005.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-TNFRSF1B antibody (ABIN3043949)

Zhou, He, Wang, Xie, Liu, Liu, Song, Ma: "Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir." in: International journal of molecular sciences, Vol. 17, Issue 6, (2017) (PubMed).

Guan, Zheng, Huang, Li, Niu, Liu: "Powerful inner/outer controlled multi-target magnetic nanoparticle drug carrier prepared by liquid photo-immobilization." in: Scientific reports, Vol. 4, pp. 4990, (2015) (PubMed).

Target Details for TNFRSF1B

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Alternative Name: Tnfrsf1b

Background:

Synonyms: Tumor necrosis factor receptor superfamily member 1B,Tumor necrosis factor receptor 2,TNF-R2,Tumor necrosis factor receptor type II,TNF-RII,TNFR-II,p75,p80 TNF-alpha receptor,CD120b,Tnfrsf1b,Tnfr-2, Tnfr2,

Tissue Specificity: Ubiquitously expressed. Expressed in skin endothelial cells, alveolar type 2 pneumocytes, bronchial epithelium and alveolar macrophages. .

Background: Tumor necrosis factor receptor 2 (TNFR2) is one of receptors of TNF. TNF has proinflammatory and immunosuppressive properties that may segregate at the level of the 2 TNF receptors (TNFRs), TNFR1 and TNFR2. The genes for TNFR1, a 55- kDa protein, and TNFR2, a 70- kDa protein, have been mapped to human chromosomes 12 (12pter-cen) and 1 (1pter-p32), respectively. TNFR2 was induced on glomerular endothelial cells of nephritic kidneys, and TNFR2 expression on intrinsic cells, but not leukocytes, was essential for glomerulonephritis and glomerular complement deposition. TNFR1 promotes systemic immune responses and renal T cell death, while intrinsic cell TNFR2 plays a critical role in complement-dependent tissue injury. Therefore, therapeutic blockade specifically of TNFR2 may be a promising strategy in the treatment of immune-mediated glomerulonephritis.

Sequence Similarities: Contains 4 TNFR-Cys repeats.

Molecular Weight: 75 kDa

UniProt: P25119

Pathways: NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway