ULK3 antibody (C-Term)

Catalog No. ABIN3043954

This Rabbit Polyclonal antibody specifically detects ULK3 in WB, IHC, FACS, ICC and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ULK3 antibody (C-Term) (ABIN3043954)

Target: ULK3 (Unc-51-Like Kinase 3 (ULK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ULK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ULK3 Antibody

Binding Specificity: C-Term

Purpose: Anti-ULK3 Antibody

Sequence: HTEVQNLMAR AEYLKEQVKM RESRWEAD

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse,rat sequences by four amino acids.

Characteristics: Anti-ULK3 Antibody. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at C-terminus of human ULK3, different from the related mouse and rat sequences by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human Immunocytochemistry, 0.5-1 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ULK3

Target: ULK3 (Unc-51-Like Kinase 3 (ULK3))

Alternative Name: ULK3

Background:

Background: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. In search of potential homologues to Drosophila and human Fu, people have cloned human serine/threonine kinase ULK3 and assessed its ability to regulate GLI transcription factors, mediators of SHH signaling. And ULK3 enhances endogenous and over-expressed GLI1 and GLI2 transcriptional activity in cultured cells, as assessed by GLI-luciferase reporter assay. Besides that, ULK3 alters subcellular localization of GLI1, as assessed by immunofluorescent staining and immunoblotting assays. It is showed that ULK3 is an autophosphorylated kinase and phosphorylates GLI proteins in vitro. Also, ULK3 catalytical activity is crucial for its function in SHH pathway. It is demonstrated that ULK3 is widely expressed and its expression is higher in a number of tissues where Shh signaling is known to be active. The data suggest that serine/threonine kinase ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins.

Gene Full Name: unc-51 like kinase 3

Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Molecular Weight: 53 kDa

Gene ID: 25989

UniProt: Q6PHR2