ABCB1 antibody (Middle Region)

Catalog No. ABIN3043967

The Rabbit Polyclonal anti-ABCB1 antibody has been validated for IHC. It is suitable to detect ABCB1 in samples from Human, Mouse and Rat. There are 30+ publications available.

Quick Overview for ABCB1 antibody (Middle Region) (ABIN3043967)

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCB1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-ABCB1 Antibody

Binding Specificity: AA 621-650, Middle Region

Purpose: Anti-P Glycoprotein/ABCB1 Antibody

Sequence: IYFKLVTMQT AGNEVELENA ADESKSEIDA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-P Glycoprotein/ABCB1 Antibody (ABIN3043967). Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human P Glycoprotein, different from the related rat sequence by twelve amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
1. Aller SG, Yu J, Ward A, Weng Y, Chittaboina S, Zhuo R, Harrell PM, Trinh YT, Zhang Q, Urbatsch IL, Chang G (March 2009). 2. Ueda K, Clark DP, Chen CJ, Roninson IB,Gottesman MM, Pastan I (January 1987). "The human multidrug resistance (mdr1) gene. cDNA cloning and transcription initiation". J. Biol. Chem.262 (2): 505-8.

Comment:

Antibody can be supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-ABCB1 antibody (ABIN3043967)

Liu, Kuang, Wu, Jin, Sun: "A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo." in: Scientific reports, Vol. 6, pp. 26722, (2018) (PubMed).

Feng, Yan, Zhou, Liang, Liang, Zhao, Dong, Ling: "Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis." in: Oncotarget, Vol. 7, Issue 40, pp. 64575-64588, (2018) (PubMed).

Duan, Di: "Acetazolamide Suppresses Multi-Drug Resistance-Related Protein 1 and P-Glycoprotein Expression by Inhibiting Aquaporins Expression in a Mesial Temporal Epilepsy Rat Model." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 5818-5825, (2018) (PubMed).

Liu, Wang, Kuang, Cao, Bao, Liu, Liu, Sun: "The natural compound GL22, isolated from Ganoderma mushrooms, suppresses tumor growth by altering lipid metabolism and triggering cell death." in: Cell death & disease, Vol. 9, Issue 6, pp. 689, (2018) (PubMed).

Deng, Li, Lin, Huang, Zeng, Wang, Li, Jin, Zhang, Li, Chen, Wang, Huang, Shao, Xu, Mao: "P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3." in: Theranostics, Vol. 6, Issue 2, pp. 204-18, (2017) (PubMed).

Ren, Qiu, Lü, Ru, Li, Xiang, Yu, Zhang: "TALENs-directed knockout of the full-length transcription factor Nrf1α that represses malignant behaviour of human hepatocellular carcinoma (HepG2) cells." in: Scientific reports, Vol. 6, pp. 23775, (2017) (PubMed).

Wang, Xi, Jiang, Ji, Yu, Zhu, Zhang: "Metronomic chemotherapy remodel cancer-associated fibroblasts to decrease chemoresistance of gastric cancer in nude mice." in: Oncology letters, Vol. 14, Issue 6, pp. 7903-7909, (2017) (PubMed).

Li, Tan, Chen, Xu, Yang, Ren, Guo, Wang, Chen, Li, Wang: "MDM4 overexpressed in acute myeloid leukemia patients with complex karyotype and wild-type TP53." in: PLoS ONE, Vol. 9, Issue 11, pp. e113088, (2016) (PubMed).

Wei, Wang, Yu, Ye, Jiang, Cheng: "Expression of TP53, BCL-2, and VEGFA Genes in Esophagus Carcinoma and its Biological Significance." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 3016-22, (2016) (PubMed).

Han, Liu, Li, Tian, Han, Wang, Fu, Guo, Zhu, Du, Tian: "HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142387, (2016) (PubMed).

Zhang, Shang, Hao, Zheng, Li, Liang, Cui, Liu: "Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats." in: American journal of translational research, Vol. 7, Issue 11, pp. 2176-86, (2016) (PubMed).

Yin, Jiang, Peng, Cui, Zhou, He, Zuo, Ouyang, Fan, Fang: "The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum." in: Oncotarget, Vol. 7, Issue 24, pp. 35592-35606, (2016) (PubMed).

Sasaki, Nitta, Sato, Nakanuma: "Autophagy may occur at an early stage of cholangiocarcinogenesis via biliary intraepithelial neoplasia." in: Human pathology, Vol. 46, Issue 2, pp. 202-9, (2015) (PubMed).

Liang, Peng, Li, Yang, Xie, Li, Du, Zhang: "Silencing of CXCR4 sensitizes triple-negative breast cancer cells to cisplatin." in: Oncotarget, Vol. 6, Issue 2, pp. 1020-30, (2015) (PubMed).

Luo, Zhou, Yi, Yi: "Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer." in: Oncology letters, Vol. 9, Issue 5, pp. 2409-2413, (2015) (PubMed).

Guo, Cui, Peng, Fang, Zuo, Deng, Wang, Wu, Chen, Deng: "Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney." in: Oncotarget, Vol. 6, Issue 34, pp. 35964-77, (2015) (PubMed).

Wang, Hou, Sun, Zhao, Tang, Hu, Yang, Zeng, Yang, Cui, Liu: "c-Ski activates cancer-associated fibroblasts to regulate breast cancer cell invasion." in: Molecular oncology, Vol. 7, Issue 6, pp. 1116-28, (2014) (PubMed).

Li, Wang, Li, Wang, Wang, Li, Han, Wang, Ma, Wang: "Proteomics analysis of normal and senescent NG108-15 cells: GRP78 plays a negative role in cisplatin-induced senescence in the NG108-15 cell line." in: PLoS ONE, Vol. 9, Issue 3, pp. e90114, (2014) (PubMed).

Lu, Luo, Wen: "Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms." in: Experimental and therapeutic medicine, Vol. 8, Issue 1, pp. 169-174, (2014) (PubMed).

Li, Wu, Wei, Wang, Sun, Li, Zhang, Zhang, Xin: "Anti-tumor effect of cactus polysaccharides on lung squamous carcinoma cells (SK-MES-1)." in: African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines, Vol. 11, Issue 5, pp. 99-104, (2014) (PubMed).

Target Details for ABCB1

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Alternative Name: ABCB1

Background:

Synonyms: Multidrug resistance protein 1,3.6.3.44,ATP-binding cassette sub-family B member 1,P-glycoprotein 1,CD243,ABCB1,MDR1, PGY1,

Tissue Specificity: Expressed in liver, kidney, small intestine and brain.

Background: P-GP, also called ABCB1 or PGY1, is a glycoprotein that in humans is encoded by the ABCB1 gene. It is mapped to 7q21.12. P-GP is a well-characterized ABC-transporter (which transports a wide variety of substrates across extra- and intracellular membranes) of the MDR/TAP subfamily. It is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent drug efflux pump with broad substrate specificity. P-GP is an ATP-dependent drug efflux pump forxenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

Sequence Similarities: Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Molecular Weight: 39 kDa

Gene ID: 5243

UniProt: P08183