MBD4 antibody (C-Term)

Catalog No. ABIN3044062

Product Details anti-MBD4 Antibody

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Binding Specificity: AA 566-580, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD4 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Anti-MBD4 Antibody Picoband®

Sequence: YHDWLWENHE KLSLS

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MBD4 Antibody (ABIN3044062). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Hendrich, B., Abbott, C., McQueen, H., Chambers, D., Cross, S., Bird, A. Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes. Mammalian Genome 10: 906-912, 1999. 2. Hendrich, B., Bird, A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Molec. Cell. Biol. 18: 6538-6547, 1998. 3. Riccio, A., Aaltonen, L. A., Godwin, A. K., Loukola, A., Percesepe, A., Salovaara, R., Masciullo, V., Genuardi M., Paravatou-Petsotas, M., Bassi, D. E., Ruggeri, B. A., Klein-Szanto, A. J. P., Testa, J. R., Neri, G., Bellacosa, A. The DNA repair gene MBD4 (MED1) is mutated in human carcinomas with microsatellite instability. (Letter) Nature Genet. 23: 266-268, 1999.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MBD4

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Alternative Name: MBD4 (MBD4 Products)

Background:

Synonyms: Methyl-CpG-binding domain protein 4,3.2.2.-,Methyl-CpG-binding endonuclease 1,Methyl-CpG-binding protein MBD4,Mismatch-specific DNA N-glycosylase,MBD4,MED1,

Tissue Specificity: Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes. .

Background: MBD4 (Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells (Hendrich and Bird, 1998). Riccio et al. (1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH. Hendrich and Bird (1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al. (1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.

Sequence Similarities: Contains 1 MBD (methyl-CpG-binding) domain.

Molecular Weight: 80 kDa

UniProt: O95243

Pathways: DNA Damage Repair