SMAD2 antibody (N-Term)

Catalog No. ABIN3044112

Cited in 2+ publications. The Rabbit Polyclonal anti-SMAD2 antibody (ABIN3044112) specifically detects SMAD2 in WB, IHC and ICC. The antibody is reactive with Human samples.

Quick Overview for SMAD2 antibody (N-Term) (ABIN3044112)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-SMAD2 Antibody

Binding Specificity: N-Term

Purpose: Anti-Smad2 Antibody

Sequence: DQWDTTGLYS FSEQTRSLD

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Characteristics: Anti-Smad2 Antibody. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Smad2, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunocytochemistry , 0.5-1 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-SMAD2 antibody (ABIN3044112)

Xu, Xu, Saud, Lu, Liu, Fang, Zhang, Hu, Li: "Effect of Kuijie Granule on the Expression of TGF-β/Smads Signaling Pathway in Patients with Ulcerative Colitis." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 2601830, (2016) (PubMed).

Tao, Hu, Li, Liu, Wu, Li, Fu, Wei, Luo: "Tranilast prevents the progression of chronic cyclosporine nephrotoxicity through regulation of transforming growth factor ?/Smad pathways." in: Transplantation proceedings, Vol. 43, Issue 5, pp. 1985-8, (2011) (PubMed).

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: SMAD2

Background:

Background: Smad2 (Mothers against decapentaplegic homolog 2), also known as MADR2, MADH2, SMAD family member 2 or SMAD2, is a protein that in humans is encoded by the SMAD2 gene. MAD homolog 2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. Eppert et al. (1996) mapped the MADR2 gene close to DPC4 at 18q21, a region which is frequently deleted in colorectal cancers. Riggins et al. (1996) mapped the human MADH2 gene to 18q21. Nakao et al. (1997) refined the localization of the SMAD2 gene to 18q21.1, approximately 3 Mb proximal to DPC4, by fluorescence in situ hybridization. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.

Gene Full Name: SMAD family member 2

Sequence Similarities: Belongs to the dwarfin/SMAD family.

Molecular Weight: 60 kDa

Gene ID: 4087

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus