MCM7 antibody (C-Term)

Catalog No. ABIN3044178

This anti-MCM7 antibody is a Rabbit Polyclonal antibody detecting MCM7 in WB, IHC, IF, FACS and ICC. Suitable for Human.

Quick Overview for MCM7 antibody (C-Term) (ABIN3044178)

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-MCM7 Antibody

Binding Specificity: AA 699-719, C-Term

Purpose: Anti-MCM7 Antibody Picoband®

Sequence: DEYEELNVWQ VNASRTRITF V

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MCM7 Antibody (ABIN3044178). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MCM7.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 2. Nakatsuru, S., Sudo, K., Nakamura, Y. Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication.Cytogenet. Cell Genet. 68: 226-230, 1995. 3. Petrocca, F., Visone, R., Onelli, M. R., Shah, M. H., Nicoloso, M. S., de Martino, I., Iliopoulos, D., Pilozzi, E., Liu, C.-G., Negrini, M., Cavazzini, L., Volinia, S., Alder, H., Ruco, L. P., Baldassarre, G., Croce, C. M., Vecchione, A. E2F1-regulated microRNAs impair TGF-beta-dependent cell-cycle arrest and apoptosis in gastric cancer. Cancer Cell 13: 272-286, 2008.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Alternative Name: MCM7

Background:

Synonyms: DNA replication licensing factor MCM7,3.6.4.12,CDC47 homolog,P1.1-MCM3,MCM7,CDC47, MCM2,

Background: MCM7 (Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped on 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. Petrocca et al. (2008) found that MCM7 and the precursors of microRNAs (miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.

Sequence Similarities: Belongs to the MCM family.

Molecular Weight: 80 kDa

UniProt: P33993

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA