MCM5 antibody (C-Term)

Catalog No. ABIN3044244

Product Details anti-MCM5 Antibody

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Binding Specificity: AA 720-734, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM5(MCM5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: EIQHRMQRKV LYRLK

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM5(MCM5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: minichromosome maintenance complex component 5
Protein Name: DNA replication licensing factor MCM5

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MCM5(720-734aa EIQHRMQRKVLYRLK), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Alternative Name: MCM5 (MCM5 Products)

Synonyms: 23.m06024 antibody, cdc46 antibody, xmcm5 antibody, CDC46 antibody, P1-CDC46 antibody, Afu5g02520 antibody, NCU01171.1 antibody, AA617332 antibody, AI324988 antibody, AL033333 antibody, Cdc46 antibody, Mcmd5 antibody, mCD46 antibody, mCDC46 antibody, mcm5 antibody, wu:fb34a06 antibody, CG4082 antibody, DmCDC46 antibody, DmCDC465 antibody, DmMCM5 antibody, DmMcm5 antibody, DmeMCM5 antibody, Dmel\\CG4082 antibody, MCM5 antibody, McM5 antibody, PCR4 antibody, DNA replication licensing factor MCM5 antibody, minichromosome maintenance complex component 5 S homeolog antibody, minichromosome maintenance complex component 5 antibody, DNA replication licensing factor Mcm5 antibody, DNA replication licensing factor mcm5 antibody, MCM5 minichromosome maintenance deficient 5 (S. cerevisiae) antibody, minichromosome maintenance complex component 5 L homeolog antibody, Minichromosome maintenance 5 antibody, DNA replication licensing factor mcm-5 antibody, BBOV_IV010040 antibody, mcm5.S antibody, MCM5 antibody, AFUA_5G02520 antibody, NCU01171 antibody, PVX_084615 antibody, LOC5576119 antibody, CpipJ_CPIJ008678 antibody, Bm1_24480 antibody, CMU_022890 antibody, Mcm5 antibody, mcm5 antibody, TP01_0722 antibody, mcm5.L antibody, mcm-5 antibody

Background: MCM5(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 5),also known as CDC46, is a protein that in humans is encoded by the MCM5 gene. MCM5 is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. And MCM5 is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The MCM5 gene is mapped on 22q12.3. The MCM5 gene contains 16 exons. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

Synonyms: CDC 46 antibody|CDC46 antibody|CDC46 homolog antibody|Cell division cycle 46 antibody|DNA replication licensing factor antibody|DNA replication licensing factor MCM 5 antibody|DNA replication licensing factor MCM5 antibody|MCM 5 antibody|MCM5 antibody|MCM5_HUMAN antibody|MGC5315 antibody|Minichromosome maintenance complex component 5 antibody|Minichromosome maintenance deficient(S. cerevisiae) 5 antibody|Minichromosome maintenance deficient 5 antibody|Minichromosome maintenance deficient protein 5 antibody|P1 CDC46 antibody|P1-CDC46 antibody

UniProt: P33992

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA