MCM5 antibody (AA 206-723)

Catalog No. ABIN7600583

The Mouse Monoclonal anti-MCM5 antibody (Clone 4G10) (ABIN7600583) specifically detects MCM5 in WB, IHC, IF, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MCM5 antibody (AA 206-723) (ABIN7600583)

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 4G10

Product Details anti-MCM5 Antibody

Binding Specificity: AA 206-723

Purpose: Anti-MCM5 Antibody (monoclonal, 4G10)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MCM5.Antibody (monoclonal, 4G10)-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MCM5 recombinant protein (Position: K206-H723).

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Alternative Name: MCM5

Background:

Background: DNA replication licensing factor MCM5 is a protein that in humans is encoded by the MCM5 gene. It is mapped to 22q12.3. The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

Gene Full Name: minichromosome maintenance complex component 5

Molecular Weight: 95 kDa

Gene ID: 4174

UniProt: P33992

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA