ATP2A1/SERCA1 antibody (Middle Region)

Catalog No. ABIN3044279

The Rabbit Polyclonal anti-ATP2A1/SERCA1 antibody is suitable to detect ATP2A1/SERCA1 in samples from Human, Rat and Mouse. It has been validated for WB and IHC.

Quick Overview for ATP2A1/SERCA1 antibody (Middle Region) (ABIN3044279)

Target: ATP2A1/SERCA1 (ATP2A1) (ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1 (ATP2A1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP2A1/SERCA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATP2A1/SERCA1 Antibody

Binding Specificity: AA 665-680, Middle Region

Purpose: Anti-SERCA1 ATPase/ATP2A1 Antibody

Sequence: EQREACRRAC CFARVE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SERCA1 ATPase/ATP2A1 Antibody (ABIN3044279). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SERCA1 ATPase, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ATP2A1/SERCA1

Target: ATP2A1/SERCA1 (ATP2A1) (ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1 (ATP2A1))

Alternative Name: ATP2A1

Background:

Synonyms: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1,SERCA1,SR Ca (2+)-ATPase 1,3.6.3.8,Calcium pump 1,Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform,Endoplasmic reticulum class 1/2 Ca (2+) ATPase,ATP2A1,

Tissue Specificity: Skeletal muscle, fast twitch muscle (type II) fibers.

Background: SERCA1 (SARCOPLASMIC RETICULUM Ca (2+)-ATPase 1), also called ATP2A1, is an enzyme that in humans is encoded by the ATP2A1 gene. This gene encodes one of the SERCA Ca (2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. The SERCA1 gene is mapped on 16p11.2. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Zhang et al. (1995)determined that the human ATP2A1 gene is 26 kb long and contains 23 exons, 1 of which can be alternatively spliced.Overexpression of S1T, but not full-length SERCA1, induced ER stress in HeLa cells and amplified ER stress through the PERK (EIF2AK3)-EIF2A -ATF4 -CHOP (DDIT3) pathway. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise.

Molecular Weight: 29 kDa

UniProt: O14983

Pathways: Ribonucleoside Biosynthetic Process