BRCA1 antibody (AA 1661-1863)

Catalog No. ABIN3044313

This anti-BRCA1 antibody is a Rabbit Polyclonal antibody detecting BRCA1 in WB, IHC and FACS. Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for BRCA1 antibody (AA 1661-1863) (ABIN3044313)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-BRCA1 Antibody

Binding Specificity: AA 1661-1863

Purpose: Anti-BRCA1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-BRCA1 Antibody Picoband® (ABIN3044313). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequences identity with mouse and rat BRCA1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Lee, M.-H., Lahusen, T., Wang, R.-H., Xiao, C., Xu, X., Hwang, Y.-S., He, W.-W., Shi, Y., Deng, C.-X. Yin Yang 1 positively regulates BRCA1 and inhibits mammary cancer formation. Oncogene 31: 116-127, 2012. 2. Thompson, M. E., Jensen, R. A., Obermiller, P. S., Page, D. L., Holt, J. T. Decreased expression of BRCA1 accelerates growth and is often present during sporadic breast cancer progression. Nature Genet. 9: 444-450, 1995. 3. Wang, B., Hurov, K., Hofmann, K., Elledge, S. J. NBA1, a new player in the Brca1 A complex, is required for DNA damage resistance and checkpoint control. Genes Dev. 23: 729-739, 2009.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-BRCA1 antibody (ABIN3044313)

Liu, Lai, Li, Sun, Cheng, Ge, Wang, Li, Zhang, De Felici, Dyce, Shen: "Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro." in: Cell death & disease, Vol. 8, Issue 8, pp. e2966, (2017) (PubMed).

Zhang, Zhang, Cong, Zhang: "The ectopic expression of BRCA1 is associated with genesis, progression, and prognosis of breast cancer in young patients." in: Diagnostic pathology, Vol. 7, pp. 181, (2013) (PubMed).

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background:

Synonyms: Breast cancer type 1 susceptibility protein,6.3.2.-,RING finger protein 53,BRCA1,RNF53,

Tissue Specificity: Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.

Background: BRCA1, also known as BRCC1, is a gene which mapping to 17q21.3. This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by mutation or by alterations in gene expression.

Sequence Similarities: Contains 2 BRCT domains.

Molecular Weight: 290 kDa

Gene ID: 672

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus