Apoptosis Inhibitor 5 antibody (C-Term)

Catalog No. ABIN3044316

This anti-Apoptosis Inhibitor 5 antibody is a Rabbit Polyclonal antibody detecting Apoptosis Inhibitor 5 in WB, IHC, IF, ICC and IHC (fro). Suitable for Human, Mouse and Rat.

Quick Overview for Apoptosis Inhibitor 5 antibody (C-Term) (ABIN3044316)

Target: Apoptosis Inhibitor 5 (API5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Apoptosis Inhibitor 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Apoptosis Inhibitor 5 Antibody

Binding Specificity: AA 487-504, C-Term

Purpose: Anti-Apoptosis inhibitor 5/API5 Antibody Picoband®

Sequence: KYSSNLGNFN YERSLQGK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Apoptosis inhibitor 5/API5 Antibody (ABIN3044316). Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human, Mouse
Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Hartz, P. A.Personal Communication.Baltimore, Md. 12/12/2005. 2. Tewari, M., Yu, M., Ross, B., Dean, C., Giordano, A., Rubin, R. : AAC-11, a novel cDNA that inhibits apoptosis after growth factor withdrawal. Cancer Res. 57: 4063-4069, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Apoptosis Inhibitor 5

Target: Apoptosis Inhibitor 5 (API5)

Alternative Name: API5

Background:

Synonyms: Apoptosis inhibitor 5,API-5,Antiapoptosis clone 11 protein,AAC-11,Cell migration-inducing gene 8 protein,Fibroblast growth factor 2-interacting factor,FIF,Protein XAGL,API5,MIG8,

Tissue Specificity: Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer. .

Background: Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death (apoptosis). Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

Sequence Similarities: Belongs to the API5 family.

Molecular Weight: 59 kDa

Pathways: Growth Factor Binding