HSPD1 antibody (C-Term)

Catalog No. ABIN3044334

This anti-HSPD1 antibody is a Rabbit Polyclonal antibody detecting HSPD1 in WB, IHC, IF, FACS and ICC. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 5+ publications.

Quick Overview for HSPD1 antibody (C-Term) (ABIN3044334)

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-HSPD1 Antibody

Binding Specificity: AA 503-516, C-Term

Purpose: Anti-HSP60/HSPD1 Antibody Picoband®

Sequence: YDAMLGDFVN MVEK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HSP60/HSPD1 Antibody (ABIN3044334). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of rat HSP60, different from the related human sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Cheng, M. Y., Hartl, F.-U., Martin, J., Pollock, R. A., Kalousek, F., Neupert, W., Hallberg, E. M., Hallberg, R. L., Horwich, A. L. : Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature 337: 620-625, 1989. 2. Zanin-Zhorov, A., Cahalon, L., Tal, G., Margalit, R., Lider, O., Cohen, I. R. : Heat shock protein 60 enhances CD4+CD25+ regulatory T cell function via innate TLR2 signaling. J. Clin. Invest. 116: 2022-2032, 2006.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Thimerosal, 0.01 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-HSPD1 antibody (ABIN3044334)

Li, Nan, Zhai, Wang, Si, Chang: "Molecular cloning, characterization, and expression of hsp60 in caudal fin regeneration of Misgurnus anguillicaudatus." in: Molecular and cellular biochemistry, Vol. 387, Issue 1-2, pp. 143-50, (2014) (PubMed).

Li, Zhai, Wang, Si, Wu, Chang: "Hsp60 in caudal fin regeneration from Paramisgurnus dabryanus: molecular cloning and expression characterization." in: Fish & shellfish immunology, Vol. 36, Issue 2, pp. 401-8, (2014) (PubMed).

Su, Baker, Christian, Naples, Tong, Zhang, Santha, Adeli: "Hepatic mitochondrial and ER stress induced by defective PPARα signaling in the pathogenesis of hepatic steatosis." in: American journal of physiology. Endocrinology and metabolism, Vol. 306, Issue 11, pp. E1264-73, (2014) (PubMed).

Hager, Li, Pun, Liu, Hossain, Maguire, Naples, Baker, Magomedova, Tam, Adeli, Cummins, Connelly, Ng: "Lecithin:cholesterol acyltransferase deficiency protects against cholesterol-induced hepatic endoplasmic reticulum stress in mice." in: The Journal of biological chemistry, Vol. 287, Issue 24, pp. 20755-68, (2012) (PubMed).

Cui, Du, Lu, Qiang: "Cloning of the heat shock protein 60 gene from the stem borer, Chilo suppressalis, and analysis of expression characteristics under heat stress." in: Journal of insect science (Online), Vol. 10, pp. 100, (2010) (PubMed).

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSPD1

Background:

Synonyms: 60 kDa heat shock protein, mitochondrial,60 kDa chaperonin,Chaperonin 60,CPN60,HSP-65,Heat shock protein 60,HSP-60,Hsp60,Mitochondrial matrix protein P1,Hspd1,Hsp60,

Tissue Specificity: In the arteries, prelamin-A/C accumulation is not observed in young healthy vessels but is prevalent in medial vascular smooth muscle cells (VSMCs) from aged individuals and in atherosclerotic lesions, where it often colocalizes with senescent and degenerate VSMCs. Prelamin-A/C expression increases with age and disease. In normal aging, the accumulation of prelamin-A/C is caused in part by the down-regulation of ZMPSTE24/FACE1 in response to oxidative stress. .

Background: HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.

Sequence Similarities: Belongs to the intermediate filament family.

Molecular Weight: 70-74 kDa

UniProt: P63039

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity