FGF8 antibody (C-Term)

Catalog No. ABIN3044346

Product Details anti-FGF8 Antibody

Target: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Binding Specificity: AA 163-185, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-Fibroblast growth factor 8 FGF8 Antibody Picoband®

Sequence: FMKRLPRGHH TTEQSLRFEF LNY

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Fibroblast growth factor 8 FGF8 Antibody (ABIN3044346). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human FGF8, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Tanaka, A., Miyamoto, K., Matsuo, H., Matsumoto, K., Yoshida, H. : Human androgen-induced growth factor in prostate and breast cancer cells: its molecular cloning and growth properties. FEBS Lett. 363: 226-230, 1995. 2. Yoshiura, K., Leysens, N. J., Chang, J., Ward, D., Murray, J. C., Muenke, M. : Genomic structure, sequence, and mapping of human FGF8 with no evidence for its role in craniosynostosis/limb defect syndromes. Am. J. Med. Genet. 72: 354-362, 1997. 3. Zammit, C., Coope, R., Gomm, J. J., Shousha, S., Johnston, C. L., Coombes, R. C. : Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer. Brit. J. Cancer 86: 1097-1103, 2002.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FGF8

Target: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Alternative Name: FGF8 (FGF8 Products)

Background:

Synonyms: Fibroblast growth factor 8,FGF-8,Androgen-induced growth factor,AIGF,Heparin-binding growth factor 8,HBGF-8,FGF8,AIGF,

Background: Fibroblast growth factor 8 (androgen-induced), also known as FGF8 or AIGF, is a human gene which maps to 10q24. The protein encoded by this gene are secreted proteins that interact with FGF tyrosine kinase receptors to mediate growth and development. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The temporal and spatial patterns of this gene expression suggest that FGF8 is involved in gastrulation, regionalization of the brain, and organogenesis of the limb and face as an embryonic epithelial factor. The adult expression of FGF8 is restricted to gonads, including testes and ovaries. FGF8 stimulated growth of human prostate carcinoma cells and mouse fibroblasts and mammary carcinoma cells in a dose-dependent manner. It also may play an important role in growth and patterning of limbs, face, and central nervous system. FGF8 is expressed in increased levels in breast cancer and in lactating human breast, it was also detected in human milk. A survey of other normal tissues showed that FGF8 is expressed in the proliferative cells of the skin and epithelial cells in colon, ovary, fallopian tube, and uterus. FGF8 in adults expression is restricted to the gonads. We detected rat ovary in our lab.

Sequence Similarities: Belongs to the heparin-binding growth factors family.

Molecular Weight: 272 kDa

UniProt: P55075

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis