CD34 antibody (C-Term)

Catalog No. ABIN3044374

This Rabbit Polyclonal antibody specifically detects CD34 in FACS, WB, IHC and IHC (fro). It exhibits reactivity toward Human, Rat and Mouse. It has been mentioned in 85+ publications

Quick Overview for CD34 antibody (C-Term) (ABIN3044374)

Target: CD34

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD34 Antibody

Binding Specificity: C-Term

Purpose: Anti-CD34 Antibody

Sequence: QATSRNGHSA RQHVVAD

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related mouse,rat sequences.

Characteristics: Anti-CD34 Antibody. Tested in Flow Cytometry, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD34, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.01 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CD34 antibody (ABIN3044374)

Hu, Li, Zhang, Zheng, Wang, Zhang, Zhang, Gu, Ye, Guo, Yang, Wang: "Phosphoinositide 3-Kinase (PI3K) Subunit p110δ Is Essential for Trophoblast Cell Differentiation and Placental Development in Mouse." in: Scientific reports, Vol. 6, pp. 28201, (2018) (PubMed).

Qin, Ke, Zhou, Wang, Liang, Wang, Yang, Gao, Ye, Kumar, Wang: "Metastasis-Associated Protein 1 Deficiency Results in Compromised Pulmonary Alveolar Capillary Angiogenesis in Mice." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 3932-3941, (2018) (PubMed).

Zhu, Xiong, Zhang, Qiu, Hua, Tang: "Comparison of semi-quantitative and quantitative dynamic contrast-enhanced MRI evaluations of vertebral marrow perfusion in a rat osteoporosis model." in: BMC musculoskeletal disorders, Vol. 18, Issue 1, pp. 446, (2018) (PubMed).

Ling, Wang, Tao, Zhang, Guan, You, Lu, Zhang, Chen, Wu, Qian, Liu, Xu, Chen: "Involvement of aberrantly activated HOTAIR/EZH2/miR-193a feedback loop in progression of prostate cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 159, (2018) (PubMed).

Li, Chen, Lin, Liu, Dai, Tang, Yang, Huang: "The earlier, the better: the effects of different administration timepoints of sorafenib in suppressing the carcinogenesis of VEGF in rats." in: Cancer chemotherapy and pharmacology, Vol. 81, Issue 1, pp. 207-216, (2018) (PubMed).

Chu, Zhang: "Inhibition of angiogenesis by leflunomide via targeting the soluble ephrin-A1/EphA2 system in bladder cancer." in: Scientific reports, Vol. 8, Issue 1, pp. 1539, (2018) (PubMed).

Zhang, Yuan, Wang, Shao, Liu, Firestone, Hong, Li, Xin, Li: "Experimental evidence of good efficacy and reduced toxicity with peptide-doxorubicin to treat gastric cancer." in: Oncotarget, Vol. 9, Issue 2, pp. 1957-1968, (2018) (PubMed).

Shi, Lv, Nie, Lu, Liu, Tian, Li, Zhang, Ren, Zhang, Kang: "Human nail stem cells are retained but hypofunctional during aging." in: Journal of molecular histology, Vol. 49, Issue 3, pp. 303-316, (2018) (PubMed).

Zhao, Feng, Sun, Qin, Zhang, Tan, Gao, Lau, Ma, Yang, Yu, Xu, Yi, Yi: "Cardiac-derived CTRP9 protects against myocardial ischemia/reperfusion injury via calreticulin-dependent inhibition of apoptosis." in: Cell death & disease, Vol. 9, Issue 7, pp. 723, (2018) (PubMed).

Chen, Zhou, Yao, Dai, Zhou, Wang, Zhang: "Dipalmitoylphosphatidic acid inhibits breast cancer growth by suppressing angiogenesis via inhibition of the CUX1/FGF1/HGF signalling pathway." in: Journal of cellular and molecular medicine, Vol. 22, Issue 10, pp. 4760-4770, (2018) (PubMed).

Wan, Wu, Huang, Ge, Liu: "Incomplete radiofrequency ablation accelerates proliferation and angiogenesis of residual lung carcinomas via HSP70/HIF-1α." in: Oncology reports, Vol. 36, Issue 2, pp. 659-68, (2017) (PubMed).

Gao, Chen, Gao, Wang, Sun, Yang: "CD47 deficiency in tumor stroma promotes tumor progression by enhancing angiogenesis." in: Oncotarget, Vol. 8, Issue 14, pp. 22406-22413, (2017) (PubMed).

Wan, Wu: "Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways." in: Journal of experimental & clinical cancer research : CR, Vol. 35, Issue 1, pp. 119, (2017) (PubMed).

Atala: "Re: Treatment of Diabetes Mellitus-Induced Erectile Dysfunction Using Endothelial Progenitor Cells Genetically Modified with Human Telomerase Reverse Transcriptase." in: The Journal of urology, Vol. 197, Issue 2, pp. 494, (2017) (PubMed).

Shi, Jiang, Wang, Cao, Liu, Xu, Wang, Sun, Niu: "Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device." in: Cell cycle (Georgetown, Tex.), Vol. 14, Issue 19, pp. 3058-65, (2016) (PubMed).

Wan, Wu, Zhang: "Local recurrence of small cell lung cancer following radiofrequency ablation is induced by HIF-1α expression in the transition zone." in: Oncology reports, Vol. 35, Issue 3, pp. 1297-308, (2016) (PubMed).

Zhang, Shang, Hao, Zheng, Li, Liang, Cui, Liu: "Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats." in: American journal of translational research, Vol. 7, Issue 11, pp. 2176-86, (2016) (PubMed).

Zhang, Wang, Dai, Cai, Zhan, Gang, Hu, Xia, Zhang: "Lentivirus-mediated PLCγ1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma." in: Oncotarget, Vol. 7, Issue 7, pp. 8043-54, (2016) (PubMed).

Jiang, Li, Wang, Xiao, Li, Liu, Liu: "Assessment of Vascularity in Hepatic Alveolar Echinococcosis: Comparison of Quantified Dual-Energy CT with Histopathologic Parameters." in: PLoS ONE, Vol. 11, Issue 2, pp. e0149440, (2016) (PubMed).

Miao, Guo, Li, Ma: "Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury." in: Neural regeneration research, Vol. 11, Issue 2, pp. 278-84, (2016) (PubMed).

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background:

Background: CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30 % of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Gene Full Name: CD34 Molecule

Sequence Similarities: Belongs to the CD34 family.

Molecular Weight: 105 kDa

Gene ID: 947

UniProt: P28906