ERK1 antibody (Middle Region)

Catalog No. ABIN3044377

This Rabbit Polyclonal antibody specifically detects ERK1 in WB, IHC and ICC. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 7+ publications.

Quick Overview for ERK1 antibody (Middle Region) (ABIN3044377)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-ERK1 Antibody

Binding Specificity: AA 287-301, Middle Region

Purpose: Anti-ERK1/MAPK3 Antibody Picoband®

Sequence: KTKVAWAKLF PKSDS

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ERK1/MAPK3 Antibody (ABIN3044377). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ERK1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Garcia F, Zalba G, Paez G, Encio I, de Miguel C (Apr 1999). "Molecular cloning and characterization of the human p44 mitogen-activated protein kinase gene". Genomics 50 (1): 69-78. 2. "Entrez Gene: MAPK3 mitogen-activated protein kinase 3". 3. Charest, D. L., Mordret, G., Harder, K. W., Jirik, F., Pelech, S. L. Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1. Molec. Cell. Biol. 13: 4679-4690, 1993.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-ERK1 antibody (ABIN3044377)

Han, Wu, Chang, Zhu, Xiao, Li, Zhang, Zhang: "Disulfiram inhibits TGF-β-induced epithelial-mesenchymal transition and stem-like features in breast cancer via ERK/NF-κB/Snail pathway." in: Oncotarget, Vol. 6, Issue 38, pp. 40907-19, (2016) (PubMed).

Tang, Zhang, Qi, Chen, Li, Liu, He, Li, Zu: "Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line." in: Experimental and therapeutic medicine, Vol. 9, Issue 5, pp. 1851-1856, (2015) (PubMed).

Liu, Zhu, Xiong, Zheng, Hu, Qiu: "Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 9, pp. 5750-61, (2014) (PubMed).

Ma, Wang, Chen, Chen, Wang, Aisa: "Icariin and icaritin stimulate the proliferation of SKBr3 cells through the GPER1-mediated modulation of the EGFR-MAPK signaling pathway." in: International journal of molecular medicine, Vol. 33, Issue 6, pp. 1627-34, (2014) (PubMed).

Yang, Han, Liu, Hu, Li, Zhu: "Negative pressure wound therapy is associated with up-regulation of bFGF and ERK1/2 in human diabetic foot wounds." in: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, Vol. 22, Issue 4, pp. 548-54, (2014) (PubMed).

Li, Zhu, Liu, Liu, Wang, Xiong, Shen, Hu, Zheng: "ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299." in: International journal of clinical and experimental pathology, Vol. 6, Issue 11, pp. 2460-7, (2013) (PubMed).

Hu, Feng, Tang, Zhu, Lin, Yu: "LIF upregulates expression of NK-1R in NHBE cells." in: Mediators of inflammation, Vol. 2006, Issue 5, pp. 84829, (2007) (PubMed).

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3

Background:

Synonyms: Mitogen-activated protein kinase 3,MAP kinase 3,MAPK 3,2.7.11.24,ERT2,Extracellular signal-regulated kinase 1,ERK-1,Insulin-stimulated MAP2 kinase,MAP kinase isoform p44,p44-MAPK,Microtubule-associated protein 2 kinase,p44-ERK1,MAPK3,ERK1, PRKM3,

Background: Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene.The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. MAPK3 gene is mapped to human chromosome 16 by hybrid cell panel analysis.

Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Molecular Weight: 100 kDa

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins