PIAS1 antibody (C-Term)

Catalog No. ABIN3044474

There is 1 publication for this product available. The Rabbit Polyclonal anti-PIAS1 antibody is suitable to detect PIAS1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ICC.

Quick Overview for PIAS1 antibody (C-Term) (ABIN3044474)

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-PIAS1 Antibody

Binding Specificity: C-Term

Purpose: Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody

Sequence: DTASIFGIIP DIISLD

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related mouse,rat sequences.

Characteristics: Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-PIAS1 antibody (ABIN3044474)

Scortegagna, Berthon, Settas, Giannakou, Garcia, Li, James, Liddington, Vilches-Moure, Stratakis, Ronai: "The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion." in: JCI insight, Vol. 2, Issue 23, (2019) (PubMed).

Target Details for PIAS1

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Alternative Name: PIAS1

Background:

Background: E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. On the other hand, PIAS1 also can function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.

Gene Full Name: protein inhibitor of activated STAT 1

Sequence Similarities: Belongs to the PIAS family.

Molecular Weight: 100 kDa

Gene ID: 8554

UniProt: O75925

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C