HNF1B antibody (C-Term)

Catalog No. ABIN3044475

Product Details anti-HNF1B Antibody

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Binding Specificity: AA 494-509, C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF1B antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Anti-HNF1 beta/HNF1B Antibody Picoband®

Sequence: HMAQQPFMAA VTQLQN

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HNF1 beta/HNF1B Antibody (ABIN3044475). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HNF1 beta, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Human, Rat
1. Bach, I., Mattei, M.-G., Cereghini, S., Yaniv, M. Two members of an HNF1 homeoprotein family are expressed in human liver. Nucleic Acids Res. 19: 3553-3559, 1991. 2. "Entrez Gene: TCF2 transcription factor 2, hepatic, LF-B3, variant hepatic nuclear factor" 3. Verdeguer, F., Le Corre, S., Fischer, E., Callens, C., Garbay, S., Doyen, A., Igarashi, P., Terzi, F., Pontoglio, M. A mitotic transcriptional switch in polycystic kidney disease. (Letter) Nature Med. 16: 106-110, 2010.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Alternative Name: HNF1B (HNF1B Products)

Background:

Synonyms: Hepatocyte nuclear factor 1-beta,HNF-1-beta,HNF-1B,Homeoprotein LFB3,Transcription factor 2,TCF-2,Variant hepatic nuclear factor 1,vHNF1,HNF1B,TCF2,

Tissue Specificity: Highest expression in brain, lower in heart, kidney, pancreas, placenta, lung and skeletal muscle.

Background: HNF1 homeobox B (hepatocyte nuclear factor 1 homeobox B), also known as HNF1B or transcription factor 2 (TCF2), is a human gene. It is a member of the homeodomain-containing superfamily of transcription factors. This gene is mapped to 17q12. The HNF1B protein is believed to form heterodimers with another liver-specific member of this transcription factor family, TCF1. HNF1B functions as both a classic transcriptional activator and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. HNF1B also can regulate renal tubulogenesis by controlling expression of SOC3. Mutation of HNF1B that disrupts normal function has been identified as the cause of MODY5 (Maturity-Onset of Diabetes, Type 5).

Sequence Similarities: Belongs to the HNF1 homeobox family.

Molecular Weight: 61 kDa

UniProt: P35680

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation