HNF1B antibody (C-Term)

Catalog No. ABIN3044475

Product Details anti-HNF1B Antibody

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Binding Specificity: AA 494-509, C-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Hepatocyte nuclear factor 1-beta(HNF1B) detection. Tested with Adiponectin in Human,Mouse,Rat.

Sequence: HMAQQPFMAA VTQLQN

Cross-Reactivity (Details): Predicted Cross Reactivity: human, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Hepatocyte nuclear factor 1-beta(HNF1B) detection. Tested with Adiponectin in Human,Mouse,Rat.
Gene Name: HNF1 homeobox B
Protein Name: Hepatocyte nuclear factor 1-beta

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HNF1 beta(494-509aa HMAQQPFMAAVTQLQN), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Predicted Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Predicted Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Alternative Name: HNF1B (HNF1B Products)

Synonyms: FJHN antibody, HNF-1B antibody, HNF1beta antibody, HNF2 antibody, HPC11 antibody, LF-B3 antibody, LFB3 antibody, MODY5 antibody, TCF-2 antibody, TCF2 antibody, VHNF1 antibody, HNF-1b antibody, HNF1B antibody, AI385728 antibody, AI987804 antibody, HNF-1Beta antibody, Hnf1beta antibody, Tcf-2 antibody, Tcf2 antibody, vHNF1 antibody, vHNF-1 antibody, chunp6877 antibody, hnf1b antibody, mst antibody, tcf2 antibody, vhnf1 antibody, lfb3 antibody, hnf-1beta antibody, hnf1-beta antibody, hnf1beta antibody, HNF1g antibody, wu:fc23f06 antibody, HNF1 homeobox B antibody, HNF1 homeobox Ba antibody, HNF1 homeobox B S homeolog antibody, HNF1 homeobox B L homeolog antibody, HNF1 homeobox Bb antibody, HNF1B antibody, Hnf1b antibody, hnf1ba antibody, hnf1b.S antibody, hnf1b.L antibody, hnf1bb antibody

Background: HNF1 homeobox B(hepatocyte nuclear factor 1 homeobox B), also known as HNF1B or transcription factor 2(TCF2), is a human gene. It is a member of the homeodomain-containing superfamily of transcription factors. This gene is mapped to 17q12. The HNF1B protein is believed to form heterodimers with another liver-specific member of this transcription factor family, TCF1. HNF1B functions as both a classic transcriptional activator and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. HNF1B also can regulate renal tubulogenesis by controlling expression of SOC3. Mutation of HNF1B that disrupts normal function has been identified as the cause of MODY5(Maturity-Onset of Diabetes, Type 5).

Synonyms: FJHN antibody|Hepatocyte nuclear factor 1 beta antibody|Hepatocyte nuclear factor 1-beta antibody|HNF 1B antibody|HNF 2 antibody|HNF-1-beta antibody|HNF-1B antibody|HNF1 beta antibody|HNF1 homeobox B antibody|HNF1B antibody|HNF1beta antibody|HNF2 antibody|Homeoprotein LF B3 antibody|Homeoprotein LFB3 antibody|HPC11 antibody|LF B3 antibody|LFB3 antibody|MODY 5 antibody|MODY5 antibody|TCF 2 antibody|TCF 2 protein antibody|TCF-2 antibody|TCF2 antibody|TCF2 protein antibody|Transcription factor 2 antibody|Transcription factor 2 hepatic antibody|Variant hepatic nuclear factor 1 antibody|Variant hepatic nuclear factor antibody|VHNF 1 antibody|vHNF1 antibody

UniProt: P35680

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation