DLG4 antibody (C-Term)

Catalog No. ABIN3044479

This Rabbit Polyclonal antibody specifically detects DLG4 in WB, IHC and IF. It exhibits reactivity toward Rat, Human and Mouse.

Quick Overview for DLG4 antibody (C-Term) (ABIN3044479)

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-DLG4 Antibody

Binding Specificity: AA 492-507, C-Term

Purpose: Anti-PSD95/DLG4 Antibody Picoband®

Sequence: RRVERREWSR LKAKDW

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PSD95/DLG4 Antibody (ABIN3044479). Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PSD95, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunofluorescence, 5 μg/mL, Rat
1. Colledge, M., Snyder, E. M., Crozier, R. A., Soderling, J. A., Jin, Y., Langeberg, L. K., Lu, H., Bear, M. F., Scott, J. D. Ubiquitination regulates PSD-95 degradation and AMPA receptor surface expression. Neuron 40: 595-607, 2003. 2. El-Husseini, A. E.-D., Schnell, E., Chetkovich, D. M., Nicoll, R. A., Bredt, D. S. PSD-95 involvement in maturation of excitatory synapses. Science 290: 1364-1368, 2000. 3. "Entrez Gene: DLG4 discs, large homolog 4 (Drosophila)"

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for DLG4

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Alternative Name: DLG4

Background:

Synonyms: Disks large homolog 4,Postsynaptic density protein 95,PSD-95,Synapse-associated protein 90,SAP-90,SAP90,DLG4,PSD95,

Tissue Specificity: Brain.

Background: DLG4 discs large homolog 4, also known as PSD95 or SAP-90, is a protein that in humans is encoded by the DLG4 gene. It is a member of the membrane-associated guanylate kinase (MAGUK) family. This gene is mapped to 17p13.1. DLG4 can heteromultimerize with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Overexpression of DLG4 in hippocampal neurons could drive maturation of glutamatergic synapses. DLG4 can orchestrate synaptic development and it has a role in synapse stabilization and plasticity. Ubiquitination of DLG4 through an MDM2-mediated pathway can regulate AMPA receptor surface expression during synaptic plasticity.

Sequence Similarities: Belongs to the MAGUK family.

Molecular Weight: 90-100 kDa

UniProt: P78352

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity