PPP2CA antibody (N-Term)

Catalog No. ABIN3044492

There is 1 publication for this product available. The Rabbit Polyclonal anti-PPP2CA antibody is suitable to detect PPP2CA in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for PPP2CA antibody (N-Term) (ABIN3044492)

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP2CA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PPP2CA Antibody

Binding Specificity: N-Term

Purpose: Anti-PP2A-alpha/PPP2CA Antibody

Sequence: FTKELDQWIE QLNEC

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Characteristics: Anti-PP2A-alpha/PPP2CA Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PP2A-alpha, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-PPP2CA antibody (ABIN3044492)

Tian, Qiu, Zhao, Li, Guo: "Sphingomyelinase-induced ceramide production stimulate calcium-independent JNK and PP2A activation following cerebral ischemia." in: Brain injury, Vol. 23, Issue 13-14, pp. 1073-80, (2009) (PubMed).

Target Details for PPP2CA

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Alternative Name: PPP2CA

Background:

Background: The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide.It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.

Gene Full Name: protein phosphatase 2 catalytic subunit alpha

Molecular Weight: 36 kDa

Gene ID: 5515

UniProt: P67775

Pathways: PI3K-Akt Signaling, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades