CDC25C antibody (C-Term)

Catalog No. ABIN3044504

There is 1 publication for this product available. The Rabbit Polyclonal anti-CDC25C antibody is suitable to detect CDC25C in samples from Human. It has been validated for WB.

Quick Overview for CDC25C antibody (C-Term) (ABIN3044504)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDC25C Antibody

Binding Specificity: AA 435-473, C-Term

Purpose: Anti-Cdc25C Antibody

Sequence: MHHQDHKTEL LRCRSQSKVQ EGERQLREQI ALLVKDMSP

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cdc25C Antibody (ABIN3044504). Tested in WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cdc25C, different from the related mouse sequence by ten amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-CDC25C antibody (ABIN3044504)

Lin, Zhu, Sun, Yang, Sun, Li, Cui, Zheng, Yu: "Prostate cancer cell proliferation is suppressed by microRNA-3160-5p via targeting of F-box and WD repeat domain containing 8." in: Oncology letters, Vol. 15, Issue 6, pp. 9436-9442, (2018) (PubMed).

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: CDC25C

Background:

Synonyms: M-phase inducer phosphatase 3,3.1.3.48,Dual specificity phosphatase Cdc25C,CDC25C,

Tissue Specificity: Present only in testis (at protein level). .

Background: M-phase inducer phosphatase 3?is an?enzyme?that in humans is encoded by the?CDC25C?gene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It s dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. Also, it is thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Molecular Weight: 53 kDa

Gene ID: 995

UniProt: P30307

Pathways: Cell Division Cycle, M Phase