Filaggrin antibody (AA 1-261)

Catalog No. ABIN3044525

Product Details anti-Filaggrin Antibody

Target: Filaggrin (FLG)

Binding Specificity: AA 1-261

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Filaggrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Filaggrin(FLG) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Filaggrin(FLG) detection. Tested with WB, IHC-P in Human.
Gene Name: filaggrin
Protein Name: Filaggrin

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Filaggrin recombinant protein (Position: M1-R261).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Filaggrin

Target: Filaggrin (FLG)

Alternative Name: FLG (FLG Products)

Background: In humans, profillagrin is encoded by the FLG gene, which is part of the S100 fused-type protein (SFTP) family within the epidermal differentiation complex on chromosome 1q21. The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.

Synonyms: Epidermal filaggrin antibody|FILA_HUMAN antibody|Filaggrin antibody|Filaggrin precursor antibody|Fillagrin antibody|FLG antibody| Profilaggrin antibody

Gene ID: 2312

UniProt: P20930

Pathways: Sensory Perception of Sound, Stem Cell Maintenance