Recognizes the HA (Hemagglutinin) epitope tag peptide sequence YPYDVPDYA, when fused to either the amino- or carboxyl- terminus of target proteins, including expression in many frequently used expression vectors. Has been tested against both the immunogen and recombinant proteins containing the HA sequence in ELISA and Western blotting. In Western blotting of bacterial extractshas been shown not to cross-react with any endogenous proteins.
Purification
Affinity purified
Immunogen
Keyhole limpet hemocyanin conjugated epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.
Reactivity: Tag
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Approved: ELISA, IHC, IHC-P, WB
Usage: Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Citrate buffer is recommended for this purpose.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
PBS, 0.09 sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.