Caspase 3 antibody

Catalog No. ABIN305732

The Mouse Monoclonal anti-Caspase 3 antibody is suitable to detect Caspase 3 in samples from Human and Mouse. It has been validated for WB, IHC and IHC (p).

Quick Overview for Caspase 3 antibody (ABIN305732)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 31A1067 

Product Details anti-Caspase 3 Antibody

Specificity: Detects both pro Caspase-3 (~32 kD) and the large subunit of the active/cleaved form (~14-21 kD) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this in Western blot applications.

Purification: Protein G purified

Immunogen: Full length recombinant human Caspase-3 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (2 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3 / Caspase 3

Background: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation