DNMT1 antibody (AA 637-650)

Catalog No. ABIN305735

The Mouse Monoclonal anti-DNMT1 antibody has been validated for WB, IHC, IP, ChIP and IHC (p). It is suitable to detect DNMT1 in samples from Human, Mouse, Monkey, Horse, Rabbit and Bat.

Quick Overview for DNMT1 antibody (AA 637-650) (ABIN305735)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human, Mouse, Monkey, Horse, Rabbit, Bat

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 60B1220-1

Product Details anti-DNMT1 Antibody

Binding Specificity: AA 637-650

Specificity: A synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bat, Horse, Rabbit (100%) Sheep, Hamster, Elephant, Dog, Bovine, Pig (93%).

Purification: Protein G purified

Immunogen: A synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bat, Horse, Rabbit (100%), Sheep, Hamster, Elephant, Dog, Bovine, Pig (93%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ChrIP, IHC, IHC-P (5 μg/mL), IP, WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT / DNMT1

Background: Name/Gene ID: DNMT1
Family: C5-methyltransferase

Synonyms: DNMT1, AIM, CXXC finger protein 9, CXXC9, DNA methyltransferase 1, DNMT, DNA methyltransferase HsaI, DNA MTase HsaI, HSN1E, M.HsaI, MCMT

Gene ID: 1786

NCBI Accession: NP_001370

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection