SAA1 antibody

Catalog No. ABIN305737

This Mouse Monoclonal antibody specifically detects SAA1 in ELISA, WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for SAA1 antibody (ABIN305737)

Target: SAA1 (Serum Amyloid A1 (SAA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SAA1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SAA1 Antibody

Brand: IHC-plus™

Specificity: Recognizes natural and recombinant human SAA. Does not show any cross-reactivity with other human cytokines or growth factors tested such as IL-1b, IL-8, MCAF, TGF-b and EGF.

Purification: Protein G purified

Immunogen: Highly purified recombinant human SAA, 12kD.

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.3 μg/mL), IHC, IHC-P (5 μg/mL), SwELISA, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA (capture coating): 0.3 μg/mL Detects 1.1 ng/mL of SAA in serum/plasma or 0.6 ng/mL of SAA in medium with an assay range of 0 to 80 ng/mL. Suggested substrate: TMB. Western Blot: 0.1-0.5 μg allows visualization of 100 ng/lane of recombinant human SAA. Used in sandwich ELISA as the capture antibody with LS-B2208 as the detection antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SAA1

Target: SAA1 (Serum Amyloid A1 (SAA1))

Alternative Name: SAA1 / SAA / Serum Amyloid A

Background: Name/Gene ID: SAA1

Synonyms: SAA1, Serum amyloid A protein, Serum Amyloid A, Serum amyloid A-1 protein, TP53I4, SAA, Serum amyloid A1, PIG4

Gene ID: 6288

UniProt: P02735

Pathways: Toll-Like Receptors Cascades