Cytochrome C antibody (AA 1-80)

Catalog No. ABIN305748

The Mouse Monoclonal anti-Cytochrome C antibody (Clone 7H8-2C12) (ABIN305748) specifically detects Cytochrome C in WB, IHC, ICC and IHC (p). The antibody is reactive with Human, Rat, Mouse, Horse, Pigeon, Drosophila melanogaster and Hamster samples.

Quick Overview for Cytochrome C antibody (AA 1-80) (ABIN305748)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human, Rat, Mouse, Horse, Pigeon, Drosophila melanogaster, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7H8-2C12

Product Details anti-Cytochrome C Antibody

Binding Specificity: AA 1-80

Specificity: An approx. 15 kD band is observed. This recognizes total cytochrome C which includes both apocytochrome (i. e. cytochrome in the cytosol without heme attached) and holocytochrome (i. e cytochrome in the mitochondria with heme attached).

Purification: Protein G purified

Immunogen: Synthetic peptide from pigeon CYCS / Cytochrome c.

Type of Immunogen: Synthetic peptide

Isotype: IgG2b

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Pigeon

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: CYCS / Cytochrome c

Background: Name/Gene ID: CYCS

Synonyms: CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic

Gene ID: 54205

UniProt: P99999

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity