Claudin 12 antibody (N-Term)

Catalog No. ABIN305786

The Rabbit Polyclonal anti-Claudin 12 antibody has been validated for WB, IHC, IHC (p) and IF. It is suitable to detect Claudin 12 in samples from Human and Mouse.

Quick Overview for Claudin 12 antibody (N-Term) (ABIN305786)

Target: Claudin 12 (CLDN12)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-Claudin 12 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Recognizes mouse Claudin 12 protein at ~27kD in Western blots. Cross-reactivity has been confirmed on mouse lung, colon and kidney homogenates. Recognizes human protein in IHC-P.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the N-terminal region of mouse and human Claudin 12.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (5 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunofluorescence: 5 μg/mL, performed using ethanol-fixed mouse colon. Positive control: Mouse lung, colon, and kidney homogenates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Claudin 12

Target: Claudin 12 (CLDN12)

Alternative Name: CLDN12 / Claudin 12

Background: Name/Gene ID: CLDN12
Family: Claudin

Synonyms: CLDN12, Claudin 12, Claudin-12

Gene ID: 9069

UniProt: P56749

Pathways: Cell-Cell Junction Organization